海洋科学2017，Vol.41Issue(10)：24-32,9.DOI:10.11759//hykx20170303003
脂肪营养对封闭循环水养殖道氏虹鳟生长、相关代谢酶及基因表达的影响
Effects of lipid levels on growth, fatty acid enzymatic activity,and gene expression of rainbow trout (Oncorhynchus mykiss) in a recirculating aquaculture system
摘要
Abstract
The aim of this trial was to evaluate the effects of dietary lipid levels on growth performance,fat metabolism,and gene mRNA expression of rainbow trout (Oncorhynchus mykiss) in a recirculating aquaculture system (RAS).Four isonitrogenous diets containing 45％ crude protein with increasing dietary lipid levels of 12％,15％,18％,and 21％ (denoted as groups Ⅰ,Ⅱ,Ⅲ,and Ⅳ) were fed to satiety to triplicate groups of 25 fishes (weighing 333.25 ± 20.71 g) for 77 days.Results are described as follows:First,the growth performance of fish fed group Ⅰ diet was optimal due to the highest weight gain (77.41％) and the lowest feed conversion rate (1.16) (P ＜0.05 or P ＜ 0.01);Second,fatty acid synthetase (FAS) activity was decreased with increasing dietary fat content,whereas the activities of lipolytic enzymes were increased.The FAS activity of fish fed group Ⅰ diet was significantly higher than those fed groups Ⅲ and Ⅳ diets by 7.5％ and 8.7％,respectively.The L-CPT Ⅰ activity of fish fed group Ⅰ diet was lower than that offish fed other diet groups by 13.08％-16.35％ (P ＜ 0.05).Meanwhile,the LPL activity of fish fed group Ⅳ diet was significantly highest (P ＜ 0.05).This result implies that fishes fed group Ⅰ diet improved their fat utilization rate by increasing fat synthesis and decreasing lipolysis to ensure the body's normal requirement for lipids and promote growth;Third,FAS mRNA expression in fish fed group Ⅰ diet was higher than that of fish fed other diet groups by 48.29％-55.15％ (P ＜ 0.05),whereas the changes in LPL showed an opposite pattern (P ＜ 0.05 or P ＜ 0.01).In the muscle,the FAS mRNA expression in fish fed group Ⅰ diet was higher than that of fish fed group Ⅳ diet by 66.82％ (P ＜ 0.05),and the expression of LPL was initially decreased and then increased with increasing dietary lipid levels.The preliminary finding was that FAS and LPL gene expression in the liver changed faster than that in the muscle with the increase in fat levels.Determined primarily,the Donaldsons rainbow trout (weighing about 330 g) could achieve healthy growth without excessive fat supply,and its fat nutrition requirement is no more than 12％ in RAS when the dietary protein level is 45％.关键词
脂肪/道氏虹鳟(Oncorhynchus mykiss)/生长/脂肪代谢/基因表达/封闭循环水养殖Key words
lipid/Oncorhynchus mykiss/growth/fat metabolism/gene expression/recirculating aquaculture system分类
农业科技引用本文复制引用
张静,李勇,孙国祥,赵宁宁,马骏,王顺奎..脂肪营养对封闭循环水养殖道氏虹鳟生长、相关代谢酶及基因表达的影响[J].海洋科学,2017,41(10):24-32,9.基金项目
“十二五”国家科技支撑计划项目(2011BAD13B07) （2011BAD13B07）
中国科学院院地合作项目(Y12530101L)National "Twelfth Five-Year" Plan for Science & Technology Support,No.2011BAD13B07 （Y12530101L）
Cooperation Projects of Chinese Academy of Sciences and Local Government,No.Y12530101L （）
