海洋科学2017，Vol.41Issue(10)：50-57,8.DOI:10.11759/hykx20170320003
GC-MS联合二维核磁对文蛤多糖Fr.2A组分结构的解析
The structure of Meretrix meretrix Linnaeus polysaccharide Fr.2A characterized by GC-MS and 2D NMR
摘要
Abstract
In this study,the structure of a polysaccharide is analyzed using GC-MS combined with two-dimensional NMR.A polysaccharide from the clam Meretrix meretrix Linnaeus (MMPX) was prepared using double enzymeassisted water extraction,and its purity was analyzed and characterized using GC-MS and 1D and 2D NMR.The result showed that Fr.2A,extracted and purified from M.meretrix Linnaeus,is a type of highly purified polysaccharide.The GC-MS analysis demonstrated that Fr.2A had three types of residue:1,4-Glc),T-Glc),and (1,4,6-Glc,and that the molar ratio of these residues was 5.58 ∶ 1.26 ∶ 1.0.The NMR spectrum results demonstrated that the main chain in Fr.2A was α-(1→4) with a small amount of α-(1→6) included,and that the branch was linked with less α-(1→6) and β-(1→4),which is a novel water-soluble D-glucan.关键词
GC-MS/核磁共振/多糖/结构解析Key words
GC-MS/2D NMR/polysaccharide/structural characterization分类
医药卫生引用本文复制引用
何永峰,钱建瑛,李恒,陆震鸣,许正宏,史劲松..GC-MS联合二维核磁对文蛤多糖Fr.2A组分结构的解析[J].海洋科学,2017,41(10):50-57,8.基金项目
国家海洋公益性行业科研专项经费项目(201305007) （201305007）
江苏省重大科技成果转化项目(BA2015006)National Marine public welfare industry research foundation of China,No.201305007 （BA2015006）
The transformation project for major scientific and technological achievements in Jiangsu Province,No.BA2015006 （）
