海洋科学2017，Vol.41Issue(10)：117-124,8.DOI:10.11759/hykx20170417002
企鹅珍珠贝Dmrt2基因的克隆及表达分析
Molecular cloning and expression analysis of Dmrt2 gene from Pteria penguin
摘要
Abstract
Dmrt2 (doublesex and mab-3-related transcription factor 2) is an important member of the Dmrt gene family with a highly conserved zinc finger-like DM domain and plays an important role in somite formation,organ formation,and skeletal patterning.This study was conducted to investigate the role of Dmrt2 in sex determination and gonadal development in Pteria penguin.The full-length cDNA of Dmrt2 gene was characterized from P.penguin by RACE-PCR,and real-time PCR was performed to assess the expression of Dmrt2 in different tissues and gonads of different growth periods.The results showed that the full-length cDNA of Dmrt2 was 1257 bp,including a 5'UTR of 52 bp,a 3'UTR of 254 bp,and an open reading frame of 951 bp,which encoded a deduced protein of 317 amino acids.The DM domain was from 20 to 73 amino acids.The predicted molecular weight was 36.61 ku,and the isoelectric point was 9.80.The amino acid sequence alignment showed that Dmrt2 of P.penguin shared 46.0％ and 45.7％ sequence identity with Dmrt2 of Pinctada margaritifera and Pinctada martensii,respectively.The real-time PCR results showed that Dmrt2 was constitutively expressed in the mantle,gill,digestive diverticulum,foot,testis,and ovary,except in the adductor muscle.The expression level was highest in the testis (P ＜ 0.05),followed by the foot.The expression analysis in gonads of different growth periods revealed that Dmrt2 had lower expression in early ovary and mature ovary,higher expression in early testis and emission testis,and the highest expression in mature testis (P ＜ 0.05).This study showed that ppDmrt2 might play a role in sex determination and gonadal development in P.penguin.关键词
企鹅珍珠贝(Pteria penguin)/ppDmrt2/基因克隆/表达分析/性别决定和性腺发育Key words
Pteria penguin/ppDmrt2/gene cloning/sex determination and gonadal development分类
生物科学引用本文复制引用
潘珍妮,余祥勇,王梅芳,曲炳良,陈耀辉,唐小玉,于非非..企鹅珍珠贝Dmrt2基因的克隆及表达分析[J].海洋科学,2017,41(10):117-124,8.基金项目
广东省科技发展专项(2016A020210115) （2016A020210115）
广东省渔港建设和渔业发展专项(B201601-Z08,Z2014005) （B201601-Z08,Z2014005）
广东海洋大学优秀青年教师项目(2014004) （2014004）
广东海洋大学博士科研启动项目(E15041) （E15041）
广东海洋大学创新强校重大科研项目(GDOU2016050248)Guangdong Provincial Science and Technology Program,No.2016A020210115 （GDOU2016050248）
Guangdong Marine Fishery Development Foundation No.B20160 1-Z08,No.Z2014005 （）
Outstanding Young Teacher Foundation,No.2014004 （）
Doctoral Scientific Research Foundation of Guangdong Ocean University,No.E15041 （）
Guangdong Major Project of Innovation School,No.GDOU2016050248 （）
