家畜生态学报2018，Vol.39Issue(3)：24-28,5.
伊犁马LTF基因多态性与产奶量相关性分析
Polymorphism of LTF Gene and its Correlation with Lactation Performance in Yili Horse
摘要
Abstract
This study was to study the relationship between lactotransfferrin (LTF) gene polymorphism and its relationship with lactation.17 pairs of primers were designed for exon and promoter of Yili horse LTF gene.DNA hybrid pool was used as template and PCR product was directly sequenced.PCR-RFLP method was used to detect the expression of LTF (lactotransfferrin) gene polymorphic genotype of 224 Yili horse,and analyzed the relationship between them and lactation traits based on 73 of them.The results showed one SNP was detected in the Yili horse LTF gene:c.173 G＞A,which was a missense mutation that occurred in the CDS.It can induce fifty-eighth amino acid of LTF protein mutating from arginine to lysine.Association analysis between SNP site and daily milk yield of 120d was conducted.The resuits showed that although the milk yield of each genotype was not significantly different (P＞0.05),it was found the milk production of each genotype being GG＞GA＞AA.The results can provide an identifiable ground for the Chinese dairy horse breeding efficiently.关键词
LTF基因/产奶量/多态性/伊犁马Key words
Lactotransfferrin gene/lactation performance/polymorphism/Yili horse分类
农业科技引用本文复制引用
杨坤,高源,邬明丽,蓝贤勇,雷初朝,陈宏,刘武军,党瑞华..伊犁马LTF基因多态性与产奶量相关性分析[J].家畜生态学报,2018,39(3):24-28,5.基金项目
陕西省重点研发计划一般项目(K3310217014) （K3310217014）
中国博士后科学基金(K308021505) （K308021505）
