摘要
Abstract
Objective To explore the application value of antinuclear antibody spectrum(ANAs),comple-ment(C3、C4)and immunoglobulin(IgG,IgM,IgA)in diagnosis of systemic lupus erythematosus(SLE).Meth-ods A total of 131 patients of SLE were collected in the experimental group,and 59 cases of other autoim-mune disease(AID)and 60 healthy persons in the control groups.The antinuclear antibody(ANA)was tested by indirect immunofluorescence(IIF)method,the antinuclear antibody spectrum was tested by the linear west-ern blot method,and serum complement and immunoglobulin levels was measured by rate nephelometry assay. The better markers for combined detection of SLE were selected.Results The positive rates of the antibodies against ANA,RNP/Sm,Sm,Ro-52,SSA,dsDNA,Nucleosome,Rib-P,C3 and C4 were 97.71%,47.33%, 22.90%,59.54%,57.25%,21.37%,19.08%,32.82%,53.44%,64.12% in SLE group respectively,which were significantly higher than those in the other AID group and the healthy control group(P< 0.05).Com-bined dsDNA and Sm with Ro-52,SSA,Nucleosome,Rib-P,C3 and C4 respectively.The positive rate of each combined detection mode in SLE group was remarkably higher than the other AID group(P<0.05).Besides, the positive rate of the mode contained Ro-52,SSA,Rib-P,complement(C3 and C4)respectively was higher than the combined mode of dsDNA and Sm alone(P<0.05).Conclusion The antibodies against Sm,Ro-52, SSA,dsDNA,Nucleosome,Rib-P,C3 and C4 have important diagnostic value for SLE.In addition,the com-bined detection mode could significantly improve the detection rate of SLE.关键词
系统性红斑狼疮/自身抗体/补体C3/补体C4Key words
systemic lupus spectrum/antinuclear antibody/complement C3/complement C4分类
医药卫生
