林产化学与工业2018,Vol.38Issue(2):51-58,8.DOI:10.3969/j.issn.0253-2417.2018.02.007
叶面喷施无机元素对杜仲叶中多酚类化合物的影响
Effects of Inorganic Elements Spray on the Content of Polyphenols in Eucommia ulmoides Olive Leaves
摘要
Abstract
The effect of three kinds of microelement fertilizers spray, such as iron, zinc and manganese, on the biomass of Eucommia ulmoides Olive leaves and the contents of total polyphenols, flavonoids and chlorogenic acid(CA) in leaves were studied. Quadratic saturation D-optimization design was used to carry out the foliage spray. The equation of fertilizer effect of Fe,Zn,and Mn were established by stepwise regression method,and optimized by frequency analysis method. The result showed that the effects of Fe,Zn,and Mn on E. ulmoides leaves were not linear accumulation of individual effects. There were interactions between the factors.During the fertilization process,interaction effects should be considered synthetically to find the balance point of fertilizer application. The suitable fertilizer application for leaves of E. ulmoides were FeSO4·5H2O 0.20%-0.22%,ZnSO4·7H2O 0.17%-0.21% and MnSO4·5H2O 0.18%-0.21%.Under the condition of Fe 0.21%,Zn 0.19% and Mn 0.20%, the biomass of leaves was 220 g each,content of total polyphenol was 6.212%,content of flavones was 3.611% and content of chlorogenic acid was 1.918%,which was significantly better than the control group.关键词
杜仲叶/多酚类化合物/叶面施肥Key words
Eucommia ulmoides Olive leaves/polyphenolic compounds/foliage dressing分类
化学化工引用本文复制引用
彭密军,彭胜,王翔,张琳杰..叶面喷施无机元素对杜仲叶中多酚类化合物的影响[J].林产化学与工业,2018,38(2):51-58,8.基金项目
国家自然科学基金资助项目(31660181) (31660181)
广东省科学院项目(2017GDASCX-0702) (2017GDASCX-0702)
湖南省科技厅项目(2016GK4058) (2016GK4058)