临床神经外科杂志2018，Vol.15Issue(2)：102-107,6.DOI:10.3969/j.issn.1672-7770.2018.02.005
Smad 6调控STAT3活性促进胶质瘤细胞的增殖
Smad 6 promotes gliomagenesis via regulating STAT3 transcriptional activity
摘要
Abstract
Objective To investigate the effects of Smad 6 on gliomagenesis and its mechanism.Methods The expression of Smad 6 in primary glioma cells was detected by Western blot.Stable glioma cell lines with nuclear-Smad 6 overexpression or knockdown mediated by lentivirus were established.Cell proliferation was examined by CCK8 and EdU experiments.The effect of Smad 6 on the self-renew of glioma stem-like cells was tested by tumor-sphere formation assay.Effect of Smad 6 on tumorgenesis in vivo was tested in nude mice.Luciferase reporter assay and Western blot analysis were used to examine STAT 3 transcriptional activity and its targeting proteins expression.Results Western blot and cell growth assay confirmed that Smad 6 expression was associated with cell growth of patient-derived glioma cells.Smad 6 overexpression significantly promoted cell proliferation and tumor-sphere formation.Reporter assay and Western blot assay revealed that Smad 6 enhanced the activity of STAT3.Conclusion Smad 6 that expressed in nuclei of glioma cells promotes cell proliferation and self-renew of tumor stem-like cells via enhancing the transcriptional activity of STAT3.关键词
Smad 6/信号转导和转录激活因子3/胶质瘤/增殖Key words
Smad 6/STAT3/glioma/proliferation分类
医药卫生引用本文复制引用
张锐,邹健,李正,叶涵斌,龚玲丽,冯素银,程超,姜晨,黄进,邵君飞..Smad 6调控STAT3活性促进胶质瘤细胞的增殖[J].临床神经外科杂志,2018,15(2):102-107,6.基金项目
国家自然科学基金(No.81372710) （No.81372710）
