临床神经外科杂志2018，Vol.15Issue(2)：108-111,115,5.DOI:10.3969/j.issn.1672-7770.2018.02.006
功能性垂体腺瘤细胞周期蛋白p21 Waf1/cip1和p27 Kip1的表达及其意义
Expression of p21Waf1/cip1and p27Kip1in functional pituitary adenomas
摘要
Abstract
Objective To explore the levels of p21Waf1/cip1and p27Kip1in functional pituitary adenomas,and analyse the relationship between the levels of p 21Waf1/cip1and p27Kip1and clinical features.Method Tissue microarray technology was performed among 6 cases with normal pituitaries, 36 invasive pituitary adenomas and 58 non-invasive pituitary adenomas. Immunohistochemistry experiment was used to detect the protein levels of p 21Waf1/cip1and p27Kip1.The clinicopathologic data of 94 patients was collected and followed up.Methylation-specific polymerase chain reaction and sequencing were detected among 3 normal pituitaries and 24 adenomas cases. Results High-p21Waf1/cip1level cases were featured by 8/36 and H-Score 148 ±28.4 in invasive functional adenomas,and 39/58 and 217.2 ±43.2 in non-invasive adenomas(χ2=18.017,P=0.000).High-p27Kip1level cases were featured by 10/36 and H-Score 122.1 ±31.1 in invasive functional adenomas, and 37/58 and 187.2 ±36.6 in non-invasive adenomas(χ2=9.446,P =0.002).The p27Kip1level was negative with serum prolactin levels in prolactinoma patients(r =-0.237,P<0.01).Average 33 CpG sites per sample were analyzed by using MALDI-TOF Mass array,and 7/33 CpG sites methylation levels of p27Kip1were higher than 50%.There were significant differences in 4 CpG sites between invasive specimens and non-invasive cases(P <0.01).Conclusion In functional pituitary adenomas,the levels of p21Waf1/cip1and p27Kip1are negatively correlated with the invasion,and promoter methylation influences the level of p 27Kip1.关键词
垂体腺瘤/p21Waf1/cip1/p27Kip1/甲基化/侵袭性Key words
pituitary adenomas/p21Waf1/Cip1/p27Kip1/methylation/invasion分类
医药卫生引用本文复制引用
郑淑荣,张亚卓,高华..功能性垂体腺瘤细胞周期蛋白p21 Waf1/cip1和p27 Kip1的表达及其意义[J].临床神经外科杂志,2018,15(2):108-111,115,5.基金项目
北京市自然科学基金(7162035) （7162035）
北京市卫计委高层次人才计划(2015-3-040) （2015-3-040）
