临床小儿外科杂志2018，Vol.17Issue(3)：226-230,5.DOI:10.3969/j.issn.1671-6353.2018.03.014
RhoA/ROCK信号通路在先天性巨结肠病变肠段的变化研究
Variation of RhoA/ROCK signaling pathway in colon of Hirschsprung's disease
摘要
Abstract
Objective To explore the protein expression of RhoA/ROCK signaling pathway in Hirschsprung's disease(HD)and provide new perspectives for studying colonic dysfunction in HD patients. Methods The protein expression involved in RhoA/ROCK signaling pathway was examined by Western blot in 9 HD cases aged from 6 months to 2 years.And statistical software was used for analyzing the results.Results The expression of P(Ser19)-MLC20obviously decreased in narrow segment as compared with dilated counterpart (t=2.527,P=0.035);and regarding the protein expressions involved in RhoA/ROCK signaling pathway,the ex-pression of ROCK1 obviously decreased in narrow segment as compared with dilated counterpart(t=3.104,P=0.015).The expression of ROCK2 decreased in narrow segment compared with dilated counterpart(t=2.357,P=0.046).The expression of P(Thr853)-MYPT1 also decreased in narrow segment as compared with dilated counterpart(t=2.857,P=0.028).And both had statistical significance.Conclusion RhoA/ROCK signaling pathway is down-regulated in HD.And these results may be related to colonic dysfunction of HD patients.关键词
Hirschsprung病/Rho激酶/肌细胞,平滑肌/收缩/结肠Key words
Hirschsprung Disease/Rho Kinase Signaling Pathway/Myocytes/Smooth Muscle/Con-striction/Colon引用本文复制引用
童玲,臧婧羽,袁小建,洪志华,邬文杰,陈杰,许文燮..RhoA/ROCK信号通路在先天性巨结肠病变肠段的变化研究[J].临床小儿外科杂志,2018,17(3):226-230,5.基金项目
国家自然科学基金(31671192) （31671192）
新华医院院级科研课题项目(JZPI201708) （JZPI201708）
