林业科学2018，Vol.54Issue(2)：52-59,8.DOI:10.11707/j.1001-7488.20180206
毛白杨PtoWOX4a基因过表达对次生生长的影响
Effect of Overexpression of Populus tomentosa WUSCHEL-related homeobox 4 (PtoWOX4a) on the Secondary Growth of Poplar
摘要
Abstract
[Objective] WUSCHEL-related homeobox (WOX) is a class of plant specific transcription factors.They play important roles in plant growth and development including stem cells maintaining,lateral organs development,de novo root regeneration of the explant,and other development processes.In this study,the expression patterns and phenotypic traits were analyzed to explore the gene function of PtoWOX4a and also to provide a basis for comprehensive research on the mechanism of WOX genes in different species.[Method] The relative expression of PtoWOX4a in shoot apical meristem,young leaf,mature leaf,tender stem,old stem,phloem,xylem and root in Populus tomentosa were analyzed by qRT-PCR.GUS staining was further analyzed the expression patterns of PtoWOX4a in different tissues.The genetic effects of PtoWOX4a on plant development were explored by studying adventitious root (AR),stem and leaf morphology and histological anatomy structure in the PtoWOX4a overexpression poplar.[Result] qRT-PCR result showed that the highest accumulation of PtoWOX4a transcripts was observed in stem,followed by root and leaf.The result of GUS staining was consistent with the qRT-PCR result,revealing that PtoWOX4a was mainly expressed in the vascular tissue of AR,leaf and stem.In 3-week-old seedlings,overexpression of PtoWOX4a shortened the AR length to 3-4 cm,which was only 1/3-1/2 compared with the control;overexpression of PtoWOX4a increased AR diameter and promoted xylem cell differentiation in AR.In 3-month-old plants,the overexpression of PtoWOX4a transgenic plants showed dwarf phenotype.Compared with the control,they exhibited 14％-20％ decrease in stem height,16％-22％ decrease in internode number.In the tenth internode,the transgenic plants showed 10％-20％ decrease in the width of pith parenchyma cells,24-％-35％ increase in the width of xylem,but the width of cambium did not change significantly.Furthermore,overexpression of PtoWOX4a caused the upward leaf curling.[Conclusion] PtoWOX4a mainly expresses in the vascular tissue of poplar.Overexpression of PtoWOX4a affects the development of vascular tissues of poplar root,leaf and stem.关键词
毛白杨/PtoWOX4a/基因表达/次生生长/解剖结构Key words
Populus tomentosa/PtoWOX4a/gene expression/secondary growth/anatomical structure分类
农业科技引用本文复制引用
李建波,贾会霞,张进,刘伯斌,胡建军,王丽娟,卢孟柱..毛白杨PtoWOX4a基因过表达对次生生长的影响[J].林业科学,2018,54(2):52-59,8.基金项目
中央级公益性科研院所基本科研业务费专项资金“WOX4与WUSa基因在杨树形成层分化调控中的互作机制”(CAFYBB2014ZX001-1). （CAFYBB2014ZX001-1）
