林业科学2018，Vol.54Issue(2)：119-125,7.DOI:10.11707/j.1001-7488.20180213
不同抗性马尾松接种松材线虫后针叶内化学信号物质的变化
Changes of Chemical Signals in Needles of Pinus massoniana with Different Resistance after Inoculation of Pine Wood Nematode
摘要
Abstract
[Objective] This study was to observe the trends of chemical signals when the different resistant individuals of Pinus massoniana had been inoculated with pine wood nematodes,in order to provide the theoretic support for clarifying the resistant mechanism of infected trees of P.massoniana.[Method]The high resistant and susceptible clones had been selected following a serious of breeding produce.The dynamic variation of signal moleculars including H2O2,O2,NO and Ca2+ was detected in needles of different resistant clones at 1,3,7,15 and 30 d post inoculation (dpi).[Result]The results showed that a large number of pine wood nematodes were detected in different parts of susceptible clones,while no nematodes were found in the high resistant clones.The H2 O2 content significantly increased in different resistant clones at 1,3 and 7 dpi.The H2O2 content in high resistant ones was returned to the health level after 15 dpi but the susceptible ones rose sustainably.As a result,the H2O2 content in susceptible clones was 1.77 times higher than that in resistant ones.The O2 content also significantly increased in both resistant and susceptible clones during 1 to 15 dpi.They had a same trends and no significantly differences.The high resistant clones reduced the O2 content at 3P dpi but the susceptible ones still increased it.There was no significant difference in NO content between high resistant clones and the control group during 1 to 15 dpi.It was significantly higher than control group at 30 dpi.However,the NO content in susceptible ones was significantly higher than that of the control group and high resistant ones at each time point.The variation trend of Ca2+ content was similar between different resistant clones post inoculation.They both performed in an up-down-updown trend.The Ca2+ content of high resistant clones was significantly higher than that of control group and susceptible ones at 1 dpi and 15 dpi.However,the Ca2+ content of high resistant clones maintained at an ordinary level at the other time points.The Ca2+ content of susceptible clones was significantly higher than that of control group at each point.[Conclusion] The content of H2O2,O~ and Ca2+ would increase rapidly when the pines were invaded by nematode.They could induce defensive response as signal moleculars.The high resistant clones had an effective way to control signal molecular content and finally returned to normal physiological metabolism.However,the susceptible ones could not control and the excessive ROS and Ca2+ would damage the plants.NO content was stable in the high resistant clones post inoculation.But it was accumulated at each time point in susceptible ones.The redundant NO is harmful for P.massoniana metabolism.关键词
马尾松/松材线虫/高抗/易感/信号分子Key words
Pinus massoniana/pine wood nematode/resistant/susceptible/signal molecular分类
农业科技引用本文复制引用
魏永成,刘青华,周志春,徐六一,陈雪莲,郝焰平..不同抗性马尾松接种松材线虫后针叶内化学信号物质的变化[J].林业科学,2018,54(2):119-125,7.基金项目
“十二五”国家科技支撑计划课题(2012BAD01B02) （2012BAD01B02）
国家自然科学基金项目(31470670) （31470670）
浙江省竹木农业新品种选育重大科技专项项目重点课题(2012C12908-12). （2012C12908-12）
