农业环境科学学报2018,Vol.37Issue(4):640-646,7.DOI:10.11654/jaes.2017-1193
磷镉交互作用对白骨壤幼苗体内镉的亚细胞分布和生理特性的影响
Effects of phosphorus and cadmium interaction on cadmium subcellular distribution and physiological characteristics in Avicennia marina(Forsk.)Vierh seedlings
摘要
Abstract
A hydroponic experiment was carried out to study the effects of phosphorus and cadmium interaction on cadmium subcellular dis-tribution and physiological characteristics in Avicennia marina(Forsk.)Vierh seedlings. Avicennia marina seedlings were used as the test materials.Four extraction methods;differential speed centrifugation,nitroblue tetrazolium,guaiacol,spectrophotometry and coomassie,were respectively adopted to determine the activity of SOD and POD,together with the chlorophyll and protein content.The results showed that increased Cd level in the medium caused a significant increase of Cd concentration in all fractions of leaves and roots,with most accumula-tion in the cell wall and soluble fraction. The content of Cd in roots was significantly higher than that in leaves, accounting for 65.23%~96.92%. Cellular compartmentation was one of the Cd detoxification mechanisms. Phosphorous could promote Cd transfer to the vacuole. With increasing P level, SOD, POD activities, chlorophyll and protein content also increased to alleviate the toxicity of Cd in A. marina seedlings to a certain extent,and thus increased the Cd tolerance of A.marina seedlings.关键词
磷/镉/白骨壤/亚细胞分布/生理特性Key words
phosphorus/cadmium/Avicennia marina(Forsk.)Vierh/subcellular distribution/physiological characteristics分类
资源环境引用本文复制引用
王岚,戴闽玥,严重玲..磷镉交互作用对白骨壤幼苗体内镉的亚细胞分布和生理特性的影响[J].农业环境科学学报,2018,37(4):640-646,7.基金项目
国家自然科学基金重点项目(31530008)The Key Program of the National Natural Science Foundation of China(31530008) (31530008)