农业环境科学学报2018，Vol.37Issue(4)：656-664,9.DOI:10.11654/jaes.2017-1523
铅胁迫对金丝草AsA-GSH循环及铅积累的影响
Influence of lead stress on the ascorbate-glutathione cycle and subcellular distribution in leaves and roots of P0g0natherum crinitum
摘要
Abstract
Pogonatherum crinitum,an effective plant for phytoremediation,can grow in soils polluted with heavy metals and subsequently ac-cumulate large amounts of heavy metals.In order to understand its response to heavy metal stress,a pot culture experiment was carried out at different lead concentrations.The results showed that the contents of glutathione reductase(GR),glutathione(GSH),ascorbic acid(AsA)in the leaves of P.crinitum were higher than those of the control samples under low Pb concentrations(1000 mg·kg-1),but not significantly. The contents of GR,AsA,and ascorbate peroxidase(APX)in the roots significantly increased by 41.1%,60.6%,and 74.0%,respective-ly, compared with those of the control samples.Low Pb concentrations had certain promotional effects on the total root length, root surface area,root mean diameter,and root volume.However,increasing Pb concentrations caused inhibitory effects on the plant,with the total root length,root surface area,and root volume decreasing significantly.In addition,increased subcellular damage occurred in the leaves and roots under high Pb concentrations(2000,3000 mg·kg-1).The contents of GR,GSH,and AsA in the leaves and roots of P.crinitum were high-er than those in the control samples under high Pb treatments and the Pb contents in cell wall and soluble constituent subcellular compo-nents accounted for over 69% of the total.These findings indicated that P.crinitum adapted to the Pb stress by inducing the ascorbate-glu-tathione(AsA-GSH)cycle and by changing the subcellular distribution of Pb,which showed potential application in the remediation of Pb-polluted soil.关键词
金丝草/铅胁迫/AsA-GSH循环/亚细胞分布Key words
Pogonatherum crinitum/Pb stress/ascorbate-glutathione cycle/subcellular distribution分类
资源环境引用本文复制引用
韩航,陈顺钰,赵雅曼,侯晓龙,蔡丽平,刘爱琴,周垂帆..铅胁迫对金丝草AsA-GSH循环及铅积累的影响[J].农业环境科学学报,2018,37(4):656-664,9.基金项目
福建省科技厅重点项目(2017Y0001) （2017Y0001）
国家自然科学基金项目(41401364) （41401364）
福建省中青年教师教育科研项目(JB10053)The Special Fund for Key Program of Science and Technology of Fujian Province,China(2017Y0001) （JB10053）
The National Natural Science Foundation of China(41401364) （41401364）
Project of Middle-aged and Young Teachers of Fujian Province,China(JB10053) （JB10053）
