农业环境科学学报2018，Vol.37Issue(4)：673-679,7.DOI:10.11654/jaes.2017-1495
塑化剂DEHP暴露对小鼠巨噬细胞的免疫毒性作用
Immunotoxicity of di(2-ethylhexyl)phthalate to macrophages
摘要
Abstract
To evaluate the potential immunotoxicity of di(2-ethylhexyl)phthalate(DEHP), RAW264.7 cells and peritoneal macrophages were treated with different concentrations of DEHP.Further,the phagocytosis efficiency and cytokine secretion and reactive oxygen species (ROS)production ability of RAW264.7 cells,and adherence ability and phagocytosis efficiency of peritoneal macrophages were determined. The results revealed that the phagocytosis efficiency of RAW264.7 cells treated with 1,5 mg·L-1and 10 mg·L-1DEHP was significantly in-hibited.The concentration of tumor necrosis factor(TNF)-α,interleukin(IL)-12,and IL-23 decreased significantly in the cell culture su-pernatant.Furthermore,there was a dose-effect relationship between DEHP exposure and concentration of TNF-α,IL-12,and IL-23.The production of ROS in DEHP-treated RAW264.7 cells was inhibited significantly with increase in the concentration of DEHP. Further, DEHP inhibited the adhesion ability and phagocytosis efficiency of peritoneal macrophages collected form DEHP-treated mice. These re-sults indicate that DEHP treatment can inhibit the normal function of both RAW264.7 cells and peritoneal macrophages.关键词
邻苯二甲酸二乙基己酯(DEHP)/巨噬细胞/RAW264.7细胞/免疫毒性Key words
di(2-ethylhexyl)phthalate/macrophage/RAW264.7 cell/immunotoxicity分类
资源环境引用本文复制引用
韩佳萦,苏伊玲,熊丽,耿辉..塑化剂DEHP暴露对小鼠巨噬细胞的免疫毒性作用[J].农业环境科学学报,2018,37(4):673-679,7.基金项目
国家自然科学基金项目(30972693) （30972693）
湖北省自然科学基金重点项目(2014CFA079) （2014CFA079）
中央高校基本科研业务费资助项目(CCNU16A02044)The National Natural Science Foundation of China(30972693) （CCNU16A02044）
Hubei Province Natural Science Key Foundation Project(2014CFA079) （2014CFA079）
Fundamental Research Funds for the Central Universities(CCNU16A02044) （CCNU16A02044）
