农业生物技术学报2018，Vol.26Issue(3)：429-436,8.DOI:10.3969/j.issn.1674-7968.2018.03.008
MC4R基因多态性及基因型组合与藏羊生长性状的关联分析
Association Analysis of MC4R Gene Polymorphisms and Genotype Combination with Growth Traits of Tibetan Sheep (Ovis aries)
摘要
Abstract
Melanoeortin receptor-4 gene (MC4R) belongs to the super family of G protein-coupled receptors that is implicated in the control of feeding behavior and energy homeostasis in mammals.This study aimed to explore the association of single nucleotide polymorphism (SNP) of MC4R gene with Tibetan sheep (Ovis aries) growth traits,so as to search for molecular markers related with growth traits,DNA sequencing method was applied to detect and genotype polymorphisms within MC4R gene in Tibetan sheep populations,and analyzed the associations of polymorphisms and combined genotypes with growth traits of Tibetan sheep.Results showed that 4 SNPs in MC4R gene were detected,including g.1803G＞A in exon 1,g.1261T＞C in intron 2 and two mutants (g.1803G＞A and g.1918C＞T) in 3'-untranslated region (3'UTR).Association analysis in 555 Tibetan sheep population indicated that,at g.880G＞A locus,individuals with genotype AA had larger higher body weight,body height,body length and chest circumference compared with the genotype GG (P＜0.01 or P＜0.05).At g.1261T＞C locus,the body weight of animals with TC genotype was significantly higher (P＜0.05) than that of animals with TT genotype.Moreover,the g.1803G＞A locus was significantly affected body weight (P＜0.01),and GG was the preferable genotype.The optical combined genotype of MC4R was diplotype H13H7 (GTGC-ATAC),which was more effective on growth traits than other combined genotypes (P＜0.05).These results suggested that the single nucleotide polymorphisms and combined genotypes of MC4R gene affected the growth traits of Tibetan sheep,and they could be used as candidate genes for breeding Tibetan sheep with better growth traits.关键词
藏羊/黑素皮质素受体4基因(MC4R)/生长性状/分子标记Key words
Tibetan sheep/Melanoeortin receptor-4 gene (MC4R)/Growth traits/Molecular markers分类
农业科技引用本文复制引用
赵宪林,曹少杰,王俊全..MC4R基因多态性及基因型组合与藏羊生长性状的关联分析[J].农业生物技术学报,2018,26(3):429-436,8.基金项目
陕西省教育厅专项科研计划项目(No.16JK2053)和渭南职业技术学院院级重点课题(No.WZYZ201704) （No.16JK2053）
