农业生物技术学报2018，Vol.26Issue(4)：564-575,12.DOI:10.3969/j.issn.1674-7968.2018.04.003
人工合成小麦农艺性状与SSR标记的关联分析
Association Analysis of Agronomic Traits with SSR Markers in Synthetic Hexaploid Wheat (Triticum aestivum)
摘要
Abstract
Synthetic hexaploid wheat (SHW) is known to be an excellent vehicle for transferring genetic variations especially many traits present in the D genome ofAegilops tauschii Cosson accessions (2n=2x=14,DD) to cultivated wheat (Triticum aestivum)(2n=6x=42,AABBDD) for improving its quality.The objective of this study was to identify 102 simple sequence repeat (SSR) markers associated with 4 important agronomic traits (plant height (PH),top internode length (TIL),spike number per plant (SNPP) and spike length (SL)) in a natural population (86 SHWs and 42 common wheats).A total of 660 alleles were identified;the number of alleles per locus varied from 3 to 11 with an average of 4.6.The polymorphism information content (PIC)value ranged from 0.247 7 to 0.897 1 with an average of 0.723 5.The maximum and minimum values of PIC values were WMS174 and GWM601,respectively.BARC241,GWM498a,GWM495,GWM359b,GWM273a and GWM508 had relative larger PIC values.The tested materials consisted of three subpopulations by the population structure analysis and cluster analysis,the two results were basically the same.There were slight differences,these may have been due to their complex genetic backgrounds.Additionally,the result of cluster analysis using unweighted pair-group with arithmetic mean (UPGMA) was in accordance with the model based analysis,except for V023 and V072;these were observed to cluster in another subgroup.However,there was a clear distinction between SHWs and common wheats,indicating that the genetic background of most SHW varieties varies greatly compared to common wheat varieties.Association analysis between traits and simple sequence repeat (SSR) markers was performed using the generalized linear model (GLM) approach.20 and 17 loci were found to be associated with the abovementioned traits in 2015 and 2016,respectively (P＜0.01).In 2015,10 loci were found to be associated with more than one trait.WMC702 was associated with both SNPP and SL;WMS408 was related with PH,TIL and SL;the explanation rate ranged from 0.039 8 to 0.247 2;the maximum and minimum rates were for GWM-111,and WMS-174,respectively.In 2016,12 loci were detected to be associated with more than one trait.GWM570b had significant association with both TIL and SL;GWM41 were associated with PH and TIL.The explanation rates range from 0.063 9 to 0.191 3,with the maximum and minimum values observed for GWM508 and WMS408,respectively.These tested significant markers may broaden our knowledge for how to harness genetic diversity and provide resource for further wheat molecular marker assisted breeding.关键词
人工合成六倍体小麦/SSR标记/遗传多样性/关联分析Key words
Synthetic hexaploid wheat/SSR marker/Genetic diversity/Association analysis分类
农业科技引用本文复制引用
张思妮,王震,赵丹阳,夏雪娇,翟晓光,张拓,杨二航,丁勤,马翎健..人工合成小麦农艺性状与SSR标记的关联分析[J].农业生物技术学报,2018,26(4):564-575,12.基金项目
农业部引进国际先进农业科学技术计划(948计划)项目(No.2015-Z39和No.2016-X58).陕西省统筹创新工程项目(No.2016KTCQ02-02) （948计划）
