农业生物技术学报2018，Vol.26Issue(4)：679-686,8.DOI:10.3969/j.issn.1674-7968.2018.04.015
梅花鹿GAL-1基因编码区克隆及表达检测
Coding Region Cloning and Expression Detection of GAL-1 Gene in Sika Deer (Cervus nippon)
摘要
Abstract
The Galectin-1 (GAL-1) gene in the deer antler stem cells is found to be highly expressed during antler regeneration period,indicating that it may be an important regulator for antler regeneration.To study the expression and possible biological function of GAL-1 in antler stem cells,antlerogenic periosteum and pedicle periosteum(potentiated pedicle periosteum and dormant pedicle periosteum) and facial periosteum of sika deer (Cervus nippon) were used as experimental materials.In this study,the GAL-1 gene CDS region of sika deer was cloned and bioinformatics analysis of GAL-1 was carried out using software or website.The relative expression of GAL-1 in the antler stem cells was detected by using Western-blot and qRT-PCR technique.The results showed that the GAL-1 ORF was 408 bp in length and encoded 134 amino acids.The relative molecular mass was 14.69 kD and the amino acid sequence was close to those of bovine (Bos taurus)and Ovis aries.GAL-1 was mainly distributed in the interstitial substance.It does not have transmembrane regions and glycosylation sites,and there may be two phosphorylation sites.The secondary structure of the GAL-1 protein was mainly β-sheets and random coil,and the tertiary structure was similar to that of the bovine.Expression level of GAL-1 was higher in the facial periosteum.In this study,the structural characteristics of GAL-1 protein was predicted,the expression level of GAL-1 protein in antler stem cells was detected and would provide the basic data for the further study of antler regeneration.关键词
半乳糖凝集素1/生物信息学分析/蛋白检测/鹿茸干细胞Key words
Galectin-1/Bioinformatics analysis/Protein detection/Antler stem cell分类
农业科技引用本文复制引用
赵志峰,孙红梅,张伟,刘慧,李春义..梅花鹿GAL-1基因编码区克隆及表达检测[J].农业生物技术学报,2018,26(4):679-686,8.基金项目
国家自然科学基金(No.31500792和No.31402059)和中国农业科学院科技创新工程项目(No.CAAS-ASTIP-2014-ISAPS-04) （No.31500792和No.31402059）
