日用化学工业2018，Vol.48Issue(2)：94-98,5.DOI:10.13218/j.cnki.csdc.2018.02.007
人参发酵液的活性物质分析及其功效研究
Analysis of active substances in ginseng fermentation broth and study on its efficacy
摘要
Abstract
In order to develop the application of ginseng fermentation broth in the field of cosmetics,ginseng was fermented using food-grade yeast usually used for preparation of Chinese rice wine.The fermentation broth was analyzed to determine the polysaccharides,proteins and ginsenosides in the broth.The antioxidant activity of the broth was measured by DPPH free radical scavenging experiment and hydroxyl free radical scavenging experiment,and let it act on the fibroblasts.MTIT cytotoxicity test was used to evaluate the proliferation of fibroblasts.The expression level of 12 inflammation-related genes in immortalized epidermal cells treated with ginseng fermentation broth was determined by fluorescence quantitative PCR to evaluate the anti-inflammatory effect of the ginseng fermentation broth.Results show that the ginseng fermentation broth is rich in polysaccharides.Ginseng fermentation broth with the mass concentration of 6.982 g/L,the protein and ginsenoside mass concentrations are 1.039 and 0.045 g/L,respectively.The volume fraction of 10.49％ ginseng fermentation broth can clear 50％ DPPH free radicals,and 19.62％ fermentation broth can remove 50％ hydroxyl free radicals.Fibroblast that is cultivated by 0.01％ (volume fraction) ginseng fermentation broth,the survival rate of them are even more than 100％,which will effectively promote cell proliferation.Ginseng fermentation broth can trigger inflammatory reactions,and in a short time activate and raise white blood cells to the site of inflammation to play an immune function.关键词
化妆品添加剂/人参/发酵/功效成分Key words
cosmetic additives/ginseng/fermentation/functional components分类
化学化工引用本文复制引用
虞旦,王昌涛,赵丹,张佳婵,苏宁,李萌..人参发酵液的活性物质分析及其功效研究[J].日用化学工业,2018,48(2):94-98,5.基金项目
质检公益性行业科研专项(201310132,201410019) （201310132,201410019）
