山东农业科学2018，Vol.50Issue(1)：1-8,8.DOI:10.14083/j.issn.1001-4942.2018.01.001
茶树CsWRKY3基因的克隆及表达分析
Cloning and Expression Analysis of CsWRKY3 Gene in Camellia sinensis
摘要
Abstract
WRKY transcription factor family exists widely in plants and has a wide range of regulation effects for a variety of physiological processes in plants,and occupys a very important position in plant transcription factor family.In this study,a WRKY gene,designated as CsWRKY3,was cloned from tea plants,and the open reading frame of CsWRKY3 was 1 710 bp,which encoded 569 amino acids.The results of real-time PCR showed that CsWRKY3 was highly expressed in roots,leaves and flowers,but was weakly expressed in stems and seeds.CsWRKY3 was quickly induced and expressed significantly when the leaves of tea plant were damaged by mechanical damage or eaten by the Ectropis obliqua larvae.This study showed that the CsWRKY3 was involved in defense response against insect pests of tea.关键词
WRKY转录因子/茶树/茶尺蠖/机械损伤/抗虫防御Key words
WRKY transcription factor/Camellia sinensis/Ectropis obliqua/Mechanical damage/Defense against insect pests分类
农业科技引用本文复制引用
戈林刚,叶保华,辛肇军,孙晓玲..茶树CsWRKY3基因的克隆及表达分析[J].山东农业科学,2018,50(1):1-8,8.基金项目
公益性行业(农业)科研专项(201403030) （农业）
国家自然科学基金项目(31471784,31401758) （31471784,31401758）
