山东农业科学2018，Vol.50Issue(1)：16-22,7.DOI:10.14083/j.issn.1001-4942.2018.01.003
基于EST-SSR标记的云南大叶茶资源遗专多样性分析
Genetic Diversity Analysis of Daye Tea Germplasm in Yunnan Based on EST-SSR Markers
摘要
Abstract
Abundant genetic variation is very important for improving environmental adaptability and genetic improvement of crop.In order to know the genetic diversity of Daye tea germplasm resources in Yunnan,94 tea germplasms were selected for the analysis using 28 pairs of SSR primers.A total of 128 distinctive loci were detected with the mean as 4.57;the Shannon's information index (Ⅰ) ranged from 0.91 to 1.66 with the mean as 1.30,and 0.91 and 1.66 were in the loci 5087 and 1969 respectively;the polymorphism information content (PIC) ranged from 0.476 to 0.753 with the mean as 0.64,and 0.476 and 0.753 were also in the loci 5087 and 1696 respectively;the average observed heterozygosity and the average expected heterozygosity were 0.71 and 0.70 respectively.The results of genetic differentiation analysis and cluster analysis for the source areas of 94 tea germplasms showed that genetic variation mainly existed between the germplasms from different source areas but not from the same source area,and most germplasms from the same source area distributed in different groups;there was no significant correlation between the genetic distance and the geographical distance.It was concluded that the cluster analysis and the analysis of PIC had the same results,which showed abundant genetic diversity.These results could provide research foundations for breeding,innovation and utilization of Yunnan tea germplasms.关键词
云南/大叶茶/EST-SSR/分子标记Key words
Yunnan/Daye tea/EST-SSR/Molecular marker分类
农业科技引用本文复制引用
尚卫琼,刘本英,毕晓清,段志芬,杨毅坚,李友勇,成浩,刘杰,杨兴荣,杨盛美,矣兵..基于EST-SSR标记的云南大叶茶资源遗专多样性分析[J].山东农业科学,2018,50(1):16-22,7.基金项目
农业部农作物种质资源保护与利用专项(2015NWB028) （2015NWB028）
云南省科技创新人才计划项目(2011CI068) （2011CI068）
科技部国家农作物种质资源平台(NICGR-065) （NICGR-065）
云南省科技计划项目“西双版纳苦茶资源遗传多样性研究”(2017FD206) （2017FD206）
