山东农业科学2018，Vol.50Issue(1)：23-28,6.DOI:10.14083/j.issn.1001-4942.2018.01.004
平邑甜茶外源花粉授粉效果和生殖机能研究
Study on Pollination Effect and Reproductive Function of Malus hupehensis with Foreign Pollen
摘要
Abstract
Malus hupehensis was used as the female parent,and was artificially pollinated the pollen of Hongli ornamental crabapple.Four treatments including no emasculation and pollination with no bagging (T1),emasculation and pollination with bagging (T2),emasculation and pollination after flowering with bagging (T3),emasculation and no pollination with bagging (T4) were conducted to research the differences in fruit setting rate,fruit physiological traits,offspring seedling hybrid rate and ploidy of Malus hupehensis by physical statistics,microscopic observation and flow cytometry.This study was aimed at further exploring the reproductive function of Malus hupehensis,selecting the period for high hybridization rate to increase the hybridization rate and the number of hybrid seeds,expanding the selection range,improving the breeding efficiency and providing important theoretical bases for breeding.The results showed that the seed weight increased by 8.7％ after pollination;the fruit setting rate significantly increased by 10.1％ compared with the natural pollination in October.The apomixes rate of Malus hupehensis was about 43.70％.Most of the hybrid offspring seedlings of the four treatments were triploid,and the diploid,tetraploid,pentaploid and aneuploidy were less.The tetraploid formation rate increased significantly under emasculation,pollination and bagging before flowering.In conclusion,artificial pollination was benefit for improving the fruit setting rate and promoting the seed formation.Malus hupehensis used apomixes as the main reproductive mode,and also had sexual hybridization.Pollination before flowering significantly increased the tetraploid formation rate.But it needed to be further identified that the reproductive mode of triploid offspring seedlings was sexual reproduction or apomix关键词
平邑甜茶/无融合生殖/倍性测定/荧光活性Key words
Malus hupehensis/Apomixes/Ploidy determination/Fluorescence activity分类
农业科技引用本文复制引用
高付凤,毛云飞,张佳腾,杨恒峰,王增辉,沈向..平邑甜茶外源花粉授粉效果和生殖机能研究[J].山东农业科学,2018,50(1):23-28,6.基金项目
国家科技支撑计划项目(2014BAD6B102) （2014BAD6B102）
山东省现代农业产业技术体系创新团队项目(SDAIT-06-07) （SDAIT-06-07）
现代农业产业技术体系建设项目(CARS-28) （CARS-28）
公益性行业(农业)科研专项(201303093) （农业）
