山东农业科学2018，Vol.50Issue(1)：111-114,4.DOI:10.14083/j.issn.1001-4942.2018.01.022
马铃薯Y病毒衣壳蛋白的原核表达及抗血清制备
Prokaryotic Expression of Potato virus Y Coat Protein and Preparation of Antiserum
摘要
Abstract
The coat protein (CP) gene of Potato virus Y (PVY) was amplified by RT-PCR and was cloned into prokaryotic expression vector pEHISTEV to produce recombinant plasmid pEHISTEV-PVY-CP,which was transformed into competent cells of Escherichia coli strain Rosetta,and a 35 kD fusion protein was expressed after induced by IPTG.The fusion protein was cut from the gel and used as antigen to immunize rabbits for production of polyclonal antiserum against the PVY CP.The titer of the resultant antiserum was 1∶16384 in ELISA.In Western blot assay,the antiserum showed specific positive reaction only with Nicotiana tabacum plants infected with PVY,but not with healthy Nicotiana tabacum plants or those infected with Potato virus X.The results laid bases for serological test and early warning work of PVY.关键词
马铃薯Y病毒/衣壳蛋白/原核表达/抗血清制备Key words
Potato virus Y/Coat protein/Prokaryotic expression/Antiserum preparation分类
农业科技引用本文复制引用
王玉,黄显德,魏代福,温亮,杨举田,陈秀斋,田延平..马铃薯Y病毒衣壳蛋白的原核表达及抗血清制备[J].山东农业科学,2018,50(1):111-114,4.基金项目
国家自然科学基金项目(31571984) （31571984）
国家重点研发计划项目(SQ2017ZY060102) （SQ2017ZY060102）
