山东农业科学2018，Vol.50Issue(2)：1-6,6.DOI:10.14083/j.issn.1001-4942.2018.02.001
山东栽培豆与野生豆抗裂荚基因SHAT1-5的克隆及序列分析
Cloning and Sequence Analysis of Pod Shattering Resistant Gene SHAT1-5 from Cultivated and Wild Soybeans in Shandong Province
摘要
Abstract
Pod shattering is one of the important factors affecting soybean yield.Therefore,the genetic mechanism of pod shattering trait in cultivated soybean has become a hot spot at domestic and overseas.In this research,the cultivars including Shanning 11,Shanning 17,Qihuang 34 and Lu 0305-1,and the wild germplasms including Dongying wild soybean 1 and 2 and Shandong wild soybean 1 and 2 were used as experimental materials.According to the previous reports,we cloned a pod shattering resistant gene SHAT1-5 through PCR amplification,and analyzed its DNA polymorphism.The results showed that SHAT1-5 appeared different multiples of TAA repeats at 1599 bp location in Shandong cultivated and wild soybeans,respectively.The key regulation region of about 4 kb in upstream of SHAT1-5 promoter was extremely conservative,and no deletion of 20 bp was found in Shandong cultivated soybean.These results are different from the previous reports,indicating that the pod shattering trait of Shandong cultivated soybean and wild soybean may be regulated by other genes.关键词
栽培豆/野生豆/裂荚/基因/多态性分析/启动子Key words
Cultivated soybean/Wild soybean/Pod shattering/Gene/Polymorphism analysis/Promoter分类
农业科技引用本文复制引用
林延慧,李伟,张彦威,刘赟,王彩洁,徐冉,张礼凤..山东栽培豆与野生豆抗裂荚基因SHAT1-5的克隆及序列分析[J].山东农业科学,2018,50(2):1-6,6.基金项目
山东省农业科学院农业科技创新工程项目(CXGC2016A02) （CXGC2016A02）
