山东农业科学2018，Vol.50Issue(2)：13-18,6.DOI:10.14083/j.issn.1001-4942.2018.02.003
花生杂交F1种子真伪的分子鉴定研究
Molecular Identification on True F1 Hybrid of Peanut
摘要
Abstract
Hybridization is an important part of peanut breeding.To breed new peanut varieties with special characters and resistance to diseases,the crossing between Tifrunner (susceptible to Aspergillus flavus) and GT-C20 (resistant to A.flavus),J62 (susceptible to bacterial wilt) and J04 (resistant to bacterial wilt),Weihua 10 (a high yielding variety in Shandong) and Yuhua29 (a peanut variety with black seed testa),Fenghua 1 (a high yielding variety in Shandong) and SAAS2015ID (a wild peanut variety) were conducted,respectively.Then the F1 hybrids were tested using the MITE transposons and SSR markers.The results showed that a total of 96 true hybrids were identified from 254 F1 hybrids,and the true hybrid rate was 37.79％.This study showed that the molecular markers could be used to identify the authenticity of peanut hybrids effectively.The identification of true hybrids before sowing could save time and cost for the planting of subsequent materials,and lay foundations for construction of genetic group and breeding of new varieties.关键词
花生/分子标记/微卫星/微型反向重复转座元件/杂交种鉴定Key words
Peanut/Molecular marker/Microsatellite/Miniature inverted repeat transposable element/Hybrid identification分类
农业科技引用本文复制引用
仇静静,赵钰涵,邓丽,任丽,夏晗,付春,赵传志,王兴军..花生杂交F1种子真伪的分子鉴定研究[J].山东农业科学,2018,50(2):13-18,6.基金项目
山东省自然科学基金项目(ZR2017MC005,ZR2015YL061) （ZR2017MC005,ZR2015YL061）
山东省农业良种工程项目(2016LZGC025) （2016LZGC025）
山东省农业科学院农业科技创新工程项目(CXGC2016B02) （CXGC2016B02）
