山东农业科学2018，Vol.50Issue(2)：119-123,5.DOI:10.14083/j.issn.1001-4942.2018.02.024
山东省及周边地区肉鸭源大肠杆菌流行病学调查及耐药性分析
Epidemiological Investigation and Analysis of Antibiotic Resistance of Escherichia coli from Duck in Shandong Province and Its Surrounding Areas
摘要
Abstract
This study was aimed to establish the methods for rapid separation and identification of Escherichia coli from ducks and drug susceptibility test,and screen proper antibiotics.We collected 447 histological materials including livers and brains from ducks with suspected coli bacillosis from 12 districts or cities of Shandong Province,4 cities of Hebei Provice and 4 cities of Henan Province.Based on the phoA gene,a PCR method was established for rapidly identifying Escherichia coli from ducks.By this method,300 strains of Escherichia coli were identified with the isolation rate as 67.11％.This PCR method could be used to rapidly identify Escherichia coli from ducks,and the identification efficiency was significantly improved.According to the standard of the Clinical and Laboratory Standards Institute (CLSI) and agar dilution method,the rapid detection method of 96 array antimicrobial susceptibility test was established.The results showed that the anti-bacterial rate of polymyxin sulfate and pefloxacin mesylate were higher,while that of kanamycin,ampicillin and florfenicol were relatively lower.So the optimal sensitive drugs and doses could be screened out according to the drug sensitivity test results,and the antibiotics could be used alternately.关键词
鸭/大肠杆菌/药敏试验/多重耐药Key words
Duck/Escherichia coli/Antibiotic susceptibility test/Multi drug resistance分类
农业科技引用本文复制引用
骆延波,李兰波,贾纪美,齐静,李璐璐,胡明,刘玉庆,邓旭明..山东省及周边地区肉鸭源大肠杆菌流行病学调查及耐药性分析[J].山东农业科学,2018,50(2):119-123,5.基金项目
山东省现代农业家禽产业技术体系建设经费项目(SDAIT-11-09) （SDAIT-11-09）
山东省科技发展计划项目(2013GNC11032) （2013GNC11032）
济南市高校自主创新计划项目(201401268) （201401268）
