食品与机械2018，Vol.34Issue(2)：73-78,6.DOI:10.13652/j.issn.1003-5788.2018.02.016
硅烷化衍生气相色谱法测定蔬菜、水果中乙烯利残留量
Determination of ethephon residues in vegetables and fruits bysilane derivation gas chromatography
摘要
Abstract
A silane derivatization gas chromatography (GC-FPD)de-tection method was established to determine the residual amount of ethylene in vegetables and fruits.Samples from ultrasonic extraction by methanol,liquid-liquid extraction by ether,and concentrated blow drying,were compared the derivative effects of 2 kinds of sila-nylation reagents under different derivatization conditions.After that, they were determined by gas chromatograph with flame photometric detector (P filter),external scalar method.The optimum derivation was as follows.Adding 100 μL BSTFA and 50 μL acetonitrile to the dry extract,derivation 3 5 min in the oven at 6 5 ℃.The external has good linear relationship in the concentration ranged from 10 to 5 000 μg/L,and the correlation coefficient was above 0.999,with the detection limit of 1 μg/kg and the limits of quantification of 3 μg/kg.The recovery rate of ethephon in different substrates at 3 concentrations was 84.3%~95.1%,and the relative standard devia-tion was 1.31%~3.92%.The method was simple,strong anti-inter-ference ability,sensitive and stable,and was suitable for the deter-mination of ethephon residues in vegetables and fruits.关键词
三甲基硅烷化/气相色谱/BSTFA/MSTFA/乙烯利Key words
trimethyl silanization/gas chromatography/BSTFA/MSTFA/ethephon引用本文复制引用
王杰斌,洪霞,钱滢文,柴宗龙,周鑫魁,张彦军,张亚鑫..硅烷化衍生气相色谱法测定蔬菜、水果中乙烯利残留量[J].食品与机械,2018,34(2):73-78,6.基金项目
中-马清真食品国家联合实验室食品检测平台建设(编号:1505JTCA017) （编号:1505JTCA017）
