生态学报2018，Vol.38Issue(7)：2271-2278,8.DOI:10.5846/stxb201704240735
短期氮磷配施对刨花楠细根形态及其土壤微生物的影响
Effect of adding a combination of nitrogen and phosphorus on fine root morphology and soil microbes of Machilus pauhoi seedling
摘要
Abstract
One-year old seedlings of Machilus pauhoi were used to determine the effect of adding a combination of N and P on the morphology of different orders of fine roots and soil microbes.For this,the phospholipid fatty acid (PLFA) analysis and root system scanning methods were used.The results were as follows:(1) Combined addition of N and P significantly improved (P＜0.05) specific root length and specific root area of the first-order and second-order fine roots,while it reduced specific root area of the third-order and fourth-order roots.(2) Root tissue density for the first-order and secondorder fine roots tended to decline,contrary to that for the third-order and fourth-order.(3) Root average diameter of the first-order to the fourth-order roots was significantly reduced (P＜0.05).(4) Total microbial,fungal,and bacterial biomass all showed a tendency to reduce after first increasing,all reaching the maximum when treated with N ∶P in the ratio of 10∶1.(5) Although the bacterial and fungal and actinomycetes biomass and total microbial were significantly and positively correlated with specific root length and specific root area for the first-and second-order roots,they were negatively correlated for the fourth-order roots.Although Gram-positive bacteria and fungal biomass were positive correlated with root tissue density for the third-order and the fourth-order,no significant correlations with the first-and second-order roots were observed.Root average diameter for all orders had no significant correlation with soil microbes.Our results suggest that the effect of short-term addition of a combination of N and P was the best when treated with N ∶P in the ratio of 10∶1,which can improve the efficiency of nutrient uptake of Machilus pauhoi seedlings' fine roots.Plants may adapt to N deposition by adjusting the fine root morphological characteristics.Meanwhile,the response of underground biological communities such as soil microbes,and the relationship of microbes with fine roots,to N deposition also changes,which affects C and N cycles and the nutrient flow in underground ecosystem.关键词
氮磷配施/细根形态/土壤微生物/根序/刨花楠苗木Key words
combination of N and P/fine root morphology/soil microbes/root order/Machilus pauhoi seedling引用本文复制引用
王艳,钟全林,徐朝斌,张中瑞,程栋梁..短期氮磷配施对刨花楠细根形态及其土壤微生物的影响[J].生态学报,2018,38(7):2271-2278,8.基金项目
国家自然科学基金项目(31170596,31170374,31370589) （31170596,31170374,31370589）
福建省种业创新和产业化工程项目(2014S1477-4) （2014S1477-4）
福建省科技厅重大项目(2014N5008) （2014N5008）
