现代检验医学杂志2018，Vol.33Issue(1)：19-21,24,4.DOI:10.3969/j.issn.1671-7414.2018.01.005
念珠菌类细菌样变异株基因特征初步研究
Preliminary Study on the Gene Characteristics of Oidiomycetes Mutant Strains Like Bacterial Morphology
摘要
Abstract
Objective To further explore the genetic characteristics of oidiomycetes mutant strains like bacterial morphology on the basis of the study on morphology and structure of mutated candida.Methods The standard strains of candida albicans were induced by low temperature and under the condition of low temperature and nutrient deficiency.Variation of standard strains of Candida albicans were induced by clinical antifungal drugs such as fluconazole with different concentration gradient.Fungal gene template was prepared by boiling method,sequences of 16SRNA and 18SRNA were amplified using bacteria conservative gene sequence of 16SRNA and fungal conserved gene sequence of 18SRNA,and observed and recorded the results agarose gel electrophoresis.At the same time,the amplified fragment of bacterial conservative gene 16SRNA was sequenced,and the sequence was analyzed by BLAST comparison.Results the 16SRNA sequences of candida variant were amplified positive,while the standard strain of candida albicans did not show the corresponding amplification band.Except 2 strains which showed a faint band,the other variants of the 18SRNA sequences did not amplified the target band,while the standard strains of candida albicans showed a corresponding amplification bands.Suggested that proportion of 18SRNA sequences in the genome of oidiomycetes mutant strains like bacterial morphology was not much even lack.The 16SRNA fragments amplified of oidiomycetes mutant strains like bacterial morphology did determination of DNA sequence after purification.BLAST comparison analysis,it was found that sequence of oidiomycetes mutant strains like bacterial morphology had higher similarity with bacterial sequences in the database.Conclusion Oidiomycetes mutant strains like bacterial morphology contained bacterial and a small amount of fungus conservative gene.Oidiomycetes mutant strains like bacterial morphology with original nuclear biological character are ones from eukaryotes.This study is great significance in biological evolution,especially in the evolution of prokaryotic cells and eukaryotic cells.关键词
念珠菌类细菌样变异株/基因/PCRKey words
Oidiomycetes mutant strains like bacterial morphology/genes/PCR分类
医药卫生引用本文复制引用
王华,苍金荣,王曦,刘家云,任健康,苏宝凤,张利侠,闫福堂,归巧娣..念珠菌类细菌样变异株基因特征初步研究[J].现代检验医学杂志,2018,33(1):19-21,24,4.基金项目
陕西省自然科学基础研究计划项目(S2014JC8839). （S2014JC8839）
