特产研究2018，Vol.40Issue(1)：17-19,3.DOI:10.16720/j.cnki.tcyj.2018.01.005
牛型结核分枝杆菌ESAT-6原核表达载体的制备和蛋白纯化
The Construction of Prokaryotic Expression Vector of ESAT-6 from Mycobacterium Bovis and Protein Purification
谷晨晨 1李海涛 1朱言柱 1常彤 1苗利光 1刘艳环 1董昕瑜 1刘爱萍2
作者信息
- 1. 中国农业科学院特产研究所,长春 130112
- 2. 集安市畜牧兽医总站,吉林集安134200
- 折叠
摘要
Abstract
The research aims to construct a prokaryotic expression vector of ESAT-6 from mycobacterium bovis and get the purified target protein.The PCR products of ESAT-6 were cloned into vector pNC-HisE,and the recombinant plasmid of ESAT6-pNC-HisE was constructed.The recombinant plasmid was transformed into bacillus subtilis,the PCR result and restriction enzyme identification show that the recombinant plasmid of ESAT6-pNC-HisE is positive.The transformed cells were grown in proper medium and can perform the secretory expression without induction.The target protein can be taken from supematant by centrifugation,purification and detection.This research can provide a quick and simple mothed which can perform the high secretory expression of ESAT-6 by bacillus subtilis expression system.关键词
牛型结核分枝杆菌/ESAT-6/原核表达/纯化Key words
Mycobacterium bovis/ESAT-6/prokaryotic expression/purification分类
生物科学引用本文复制引用
谷晨晨,李海涛,朱言柱,常彤,苗利光,刘艳环,董昕瑜,刘爱萍..牛型结核分枝杆菌ESAT-6原核表达载体的制备和蛋白纯化[J].特产研究,2018,40(1):17-19,3.
