中国组织工程研究2018，Vol.22Issue(6)：902-907,6.DOI:10.3969/j.issn.2095-4344.0066
新型纳米仿生-防粘连复合型疝补片的力学性能及细胞相容性
Mechanical properties and cytocompatibility of a new-type nano-bionic anti-adhesion hernia mesh
摘要
Abstract
BACKGROUND:A new-type nano-bionic anti-adhesion hernia mesh was developed in our previous research.OBJECTIVE:To investigate the mechanical properties and cytocompatibility of the new-type nano-bionic anti-adhesion hernia mesh.METHODS:The tensile strength of the compound hernia mesh was detected using a textile detector.Mouse fibroblasts (L929) were cultured with the compound hernia mesh,and cell structures on the mesh surface were observed under electron microscope at 1,3,5 days after culture.In addition,L929 cells were co-cultured with compound hernia mesh,polypropylene patch,and polyester patch,respectively.Cells cultured alone were used as negative controls.After 1,3,5 days of culture,MTS array was used to detect cell proliferation and evaluate cytotoxicity;after 3 days of culture,western blot was used to detect the content of type Ⅰ and Ⅲ collagens.RESULTS AND CONCLUSION:The average tensile strength of the compound hernia mesh was 31.2 N The number of fibroblasts on the nanofibrous layer of the compound hernia mesh increased as long as cultured.The cells spread along the nanofibers and pseudopodia extended from the cells formed polygon and fusiform structures,with a good cross-linking with the mesh.A complete cell layer covered all pores of the nanofibers at 5 days.The cytotoxicity of the nanofibrous layer of the compound hernia mesh was graded 0,and the cytotoxicity was graded 1 of polypropylene and polyester patches.All the three kinds of patches fulfilled the implantation requirements,and the compound hernia mesh had better biological properties.No significant differences were found among groups in the contents of type Ⅰ and Ⅲ collagens at 3 days of culture.To conclude,the new-type nano-bionic anti-adhesion hernia mesh has good mechanical properties and cytocompatibility.关键词
疝补片/纳米纤维/细胞相容性/成纤维细胞/胶原/生物材料分类
医药卫生引用本文复制引用
唐骁,詹泽丰,卫洪波,叶小龙,黄江龙,杨晓峰,郑宗珩,魏波,陈图锋,黄勇,罗林波..新型纳米仿生-防粘连复合型疝补片的力学性能及细胞相容性[J].中国组织工程研究,2018,22(6):902-907,6.基金项目
广东省科技计划项产学研合作项目(2014B090901066) （2014B090901066）
广州市科技计划项目产学研协同创新重大专项(2014Y2-00503) （2014Y2-00503）
广东省自然科学基金-博士启动(2015A030310052)the University-Industry Collaboration for Science and Technology Research of Guangdong Province,No.2014B090901066 （2015A030310052）
Major Innovation Special Plan & the University-Industry Collaboration for Science and Technology Research of Guangdong Province,No.2014Y2-00503 （）
the Natural Science Foundation of Guangdong Province for Doctoral Startup Project,No.2015A030310052 （）
