新疆农业科学2017，Vol.54Issue(12)：2157-2163,7.DOI:10.6048/j.issn.1001-4330.2017.12.001
新疆部分小麦材料淀粉品质基因等位变异的分子鉴定及其分布
Molecular Identification and Distribution of Allelic Variation of Starch Quality Genes in Some Wheat Varieties in Xinjiang
摘要
Abstract
[Objective] The study is to research the allelic composition and distribution of starch quality gene (Wx-A1,Wx-B1 and Wx-D1) and the difference of starch quality gene between local wheat materials of Xinjiang and introduced wheat materials in the hope of laying the foundation for improving wheat variety in Xinjiang.[Method] Using molecular markers MAG264,BDFL/BRD and MAG269 to identify the distribution of starch quality genes Wx-A1,Wx-B1 and Wx-D1 in cultivated wheat germplasms in Xinjiang.[Result] The results showed that in the 169 materials to be identified,18.67％ materials of winter wheat had allele Wx -A1a and Wx-B1a,10.67％ had Wx-D1a,and 30.85％ materials of spring wheat had allele Wx-A1b,41.49％ had Wx-B1b.The distribution frequencies of oversea cultivars,other Chinese cultivars,Xinjiang local cultivars were respectively 74.49％,84.78％ and 56％ in mutant gene type Wx-A1a,for Wx-A1b gene type,they were respectively 25.51％,15.22％ and 44％,for Wx-B1a gene type they were respectively 63.27 ％,82.61％ and 64 ％,for Wx-B1b gene type they were respectively 36.73％,17.39 ％ and 36 ％.All foreign varieties and Xinjiang local varieties carried Wx-D1a gene,82.61％ materials of other Chinese cultivars had Wx-D1a and 17.39％ materials of other Chinese cultivars had Wx-D1b.Foreign varieties and local varieties of Xinjiang did not carry this gene.[Conclusion] The study concluded that the frequency of subunit mutation of Wx-A1 and Wx-B1 genes in 94 spring wheat was much higher than that in winter wheat and Wx-D1b gene was not tested in Xinjiang local cultivars and other Chinese cultivars.18.67％ materials of spring wheat had allele Wx-A1a and Wx-B1a gene,10.67％ had Wx-D1a.At the same time,14 varieties lost Wx-A1 and Wx-B1 gene types,among which 4 were Xinjiang local cultivars and 10 varieties were foreign ones.At the same time,three copies were of Wx-B1,Wx-D1 deletion gene type and they were all domestic cultivars.关键词
小麦/Wx基因/分子标记/等位变异Key words
wheat/Wx gene/molecular markers/alleles分类
农业科技引用本文复制引用
哈力旦·依克热木,周安定,芦静,曹俊梅,刘联正,张新忠..新疆部分小麦材料淀粉品质基因等位变异的分子鉴定及其分布[J].新疆农业科学,2017,54(12):2157-2163,7.基金项目
自治区公益性科研院所基本科研业务经费资助项目(KYGY2016117) （KYGY2016117）
新疆维吾尔自治区研究与技术开发计划重大专项(201230116-1) （201230116-1）
农业部西北地区小麦抗旱耐盐生物学科学观测实验站Basic scientific research funds of public welfare scientific research institutes in Xinjiang Uygur Autonomous Region (KYGY2016117) （KYGY2016117）
The special funds for major research and technology development plan projects of Xinjiang Uyghur Aotonomouse Region (201230116-1) （201230116-1）
Biological observation and experiment station for drought resistance and salt tolerance of wheat in Northwest China of the Ministry of Agriculture （）
