新疆农业科学2017，Vol.54Issue(12)：2232-2238,7.DOI:10.6048/j.issn.1001-4330.2017.12.010
胡杨休眠茎尖的包埋玻璃化法冷冻保存研究
Cryopreservation of Shoot-tips of Populus euphratica Dormant Buds by Encapsulation-vitrification
摘要
Abstract
[Objective] To establish appropriate cryopreservation method to preserve shoot-tips of Populus euphratica dormant buds by using encapsulation-vitrification,thus preserving the germplasm resources of the endangered rare plant.[Method] Dormant shoot-tips were encapsulated in 3％ calcium alginate beads.The main factors influencing the viability such as sucrose concentration,preculture time,different vitrification solution and the time in vitrification solution were studied.[Result] The results showed that the high viability could be obtained when alginate beads were precultured for 24 h in the 0.8 mol/L sucrose and the beads were dehydrated with vitrification solution [40％ Gly + 45％ (0.4 mol/L) sucrose + 10％ PEG (4,000) + 5％ DMSO] for 30 min at 25℃.[Conclusion] Under optimized conditions,the viability of dormant shoot-tips can reach 77％ at the most.After cryopreservation,the dormant shoot-tips can grow directly and the genetic stability can be maintained.It is feasible to freeze the dormant shoot tips of Populus euphratica by liquid nitrogen.关键词
胡杨/休眠茎尖/冷冻保存/包埋玻璃化法Key words
Populus euphratica/dormant shoot-tip/cryopreservation/encapsulation-vitrification分类
农业科技引用本文复制引用
张玲,田洪涛,刘锦涛,刘艳萍..胡杨休眠茎尖的包埋玻璃化法冷冻保存研究[J].新疆农业科学,2017,54(12):2232-2238,7.基金项目
国家自然科学基金项目“超干处理对塔里木盆地四种珍稀濒危植物种子活力影响机理的研究”(31160069) （31160069）
塔里木大学校长基金“包埋玻璃化法超低温保存塔里木盆地四种珍稀濒危植物的研究”(TDZKSS06001)National Natural Science Foundation of China" Study on the mechanism of ultra-dry preservation on seed vigor of four rare and endangered plants in Tarim Basin (31160069)" （TDZKSS06001）
Tarim University president fund" Study on cryopreservation of four rare and endangered plants in Tarim Basin by vitrification(TDZKSS06001)" （TDZKSS06001）
