畜牧兽医学报2018，Vol.49Issue(4)：675-684,10.DOI:10.11843/j.issn.0366-6964.2018.04.003
miR-142和miR-144靶向FoxO1基因调节绵羊前体脂肪细胞分化的研究
Study on the Differentiation Regulation of miR-142 and miR-144 on Ovine Preadipocytes by Targeting FoxO1 Gene
摘要
Abstract
This study aimed to predict and validate the crucial miRNAs that regulate FoxO1 gene expression,and to elucidate the functional mechanism of these miRNAs in ovine adipose differentiation.Four online softwares were used to predict the miRNAs that might target FoxO1.A dualluciferase reporter assay system was employed to detect luciferase activity.qPCR,Western blotting and Oil red O staining were used to detect the effect of these miRNAs on the expression of FoxO1 both at mRNA and protein levels,and on the regulation of ovine preadipocyte differentiation.The results showed that FoxO1 was targeted by both miR-142 and miR-144.Overexpression of miR-142 or miR-144 significantly inhibited the luciferase activity of the dual-luciferase reporter vector,and reduced the expression of FoxO1 mRNA (P＜0.01) and protein (P＜0.05).Both miR-142 and miR-144 promoted the differentiation of ovine preadipocytes and accelerated the formation of lipid droplets.In conclusion,miR-142 and miR-144 can down-regulate the expression of FoxO1,and further down-regulate PPARγ expression,thus promote the differentiation of ovine preadipocytes.关键词
miR-142/miR-144/FoxO1/基因表达/脂肪细胞分化Key words
miR-142/miR-144/FoxO1/gene expression/adipocyte differentiation分类
农业科技引用本文复制引用
赵艳艳,乔利英,景炅婕,潘洋洋,任端阳,王书芳,吕宣增,刘文忠..miR-142和miR-144靶向FoxO1基因调节绵羊前体脂肪细胞分化的研究[J].畜牧兽医学报,2018,49(4):675-684,10.基金项目
山西优势肉用家畜高效安全生产协同创新中心项目 （）
山西农业大学拔尖创新人才支持计划项目(BJRC201203) （BJRC201203）
