畜牧兽医学报2018，Vol.49Issue(4)：685-692,8.DOI:10.11843/j.issn.0366-6964.2018.04.004
Wnt10b对山羊前体脂肪细胞分化相关基因表达的影响
Effect of Wnt10b on the Expression of Precursor Adipocytes Differentiation Related Genes in Goat
摘要
Abstract
The aim of this study was to investigate the effect of Wnt10b gene on the precursor adipocytes differentiation of goat by RNA interference.The goat Wnt10b siRNA sequence was designed and compounded,and the goat subcutaneous and intramuscular precursor adipocytes were obtained by collagenase digestion.The effect of Wnt10b gene expression on the cell differentiation and adipocyte differentiation marker genes expression were detected by using the effective siRNA sequence to interfere the expression of Wnt 10b gene in goat subcutaneous and intramuscu1ar precursor adipocytes.The resu1ts showed that an effective goat Wnt10b siRNA sequence was obtained,and the expression of Wnt10b was down-regulated by 63％ (P＜0.01) and 67％ (P＜0.01),respectively after transfection in goat subcutaneous and intramuscular precursor adipocytes.Results of Oil red O staining showed that the accumulation of lipid droplets were inhibited significantly by Wnt10b RNAi in goat subcutaneous and intramuscular adipocytes.The C/EBPβ,PPARγ,SREBP1 and Pref1 genes were extremely significantly down-regulated in the subcutaneous precursor adipocyte differentiation after Wnt 10b RNAi(P＜0.01),LPL was significantly down-regulated(P＜0.05).The AP2 and LPL were extremely significantly down-regulated (P＜0.01),C/EBPβ was significantly down-regulated (P＜0.05),and Pref1 was extremely significantly up-regulated (P＜0.01) in intramuscular precursor adipocytes differentiation.In this study,it was found that Wnt 10b gene could promote the differentiation of goat subcutaneous precursor adipocytes by regulating the expression of C/EBPβ,PPARγ,SREBP1 and LPL,and promote the differentiation of goat intramuscular precursor adipocytes by regulating the expression of AP2,LPL,C/EBPβ and Pref1.关键词
山羊/Wnt10b基因/RNA干扰/皮下脂肪细胞/肌内脂肪细胞Key words
goat/Wnt10b gene/RNA interference/subcutaneous adipocytes/intramuscular adipocytes分类
农业科技引用本文复制引用
林森,林亚秋,朱江江,许晴,赵越,池永东,王永..Wnt10b对山羊前体脂肪细胞分化相关基因表达的影响[J].畜牧兽医学报,2018,49(4):685-692,8.基金项目
四川省“十三五”畜禽育种攻关项目(2016NYZ0045) （2016NYZ0045）
国家自然科学基金(31672395) （31672395）
中央高校基本科研业务费专项基金项目(2017NGJPY06) （2017NGJPY06）
