畜牧兽医学报2018，Vol.49Issue(4)：709-717,9.DOI:10.11843/j.issn.0366-6964.2018.04.007
牦牛新鲜囊胚与玻璃化冻融囊胚转录组的比较分析
Comparative Transcriptome Analysis between Fresh and Vitrified-thawed Blastocysts of the Yak (Bos grunniens)
摘要
Abstract
The study aimed to investigate the transcriptome differential expression between preand post-vitrified yak blastocysts in order to understand the effect of vitrification on the gene expression profile of yak blastocysts.Total RNAs were extracted respectively from fresh blastocysts (FRB) and vitrified-thawed blastocysts (VTB) that were in vitro-produced from yaks,and then the Smart-seq2 method was used to amplify and construct RNA libraries,and finally transcriptome was sequenced using RNA-seq.The | log2 (VTB/FRB) | ≥1 and Q-value＜0.05 were set as thresholds for identifying differentially expressed gene (DEG).The DEGs were then searched against the GO and KEGG database for enrichment analysis.The results showed that 9 827 and 13 567 transcripts were detected in FRB and VTB,respectively.A total of 11 174 DEGs were identified between the 2 libraries,in which 7 037 were up-regulated,and 4 137 were down-regulated in VTB.10 538 DEGs were significantly enriched in 479 GO terms (P＜0.05),and there were 318 pathways involved,of which 8 pathways including ribosome biogenesis in eukaryotes,spliceosome and neuroactive ligand-receptor interaction were significantly enriched (P＜0.05).In conclusion,this report analyzed the transcriptome change pre-and post-vitrification of yak blastocysts using RNA-seq,which provides a theoretical basis to explore the mechanism of vitrification damage and improve vitrification technology of yak blastocysts.关键词
牦牛/体外受精/囊胚/玻璃化冷冻/转录组测序Key words
yak/in vitro fertilization/blastocyst/vitrification/transcriptome sequencing分类
农业科技引用本文复制引用
蒲思颖,郑杰,杨远潇,王琴,杨绕芬,字向东..牦牛新鲜囊胚与玻璃化冻融囊胚转录组的比较分析[J].畜牧兽医学报,2018,49(4):709-717,9.基金项目
中央高校基本科研业务费专项资金项目(2015NZYTD02) （2015NZYTD02）
