畜牧兽医学报2018，Vol.49Issue(4)：718-724,7.DOI:10.11843/j.issn.0366-6964.2018.04.008
SOX5蛋白对公鸡精子发生和精子活力的作用及其定位
The Effect of SOX5 Protein in Spermatogenesis and Sperm Motility Regulation and Its Expression Localization in the Testis of Roosters
摘要
Abstract
The aim of the present study was to investigate the expression of SOX5 in the testis of chickens and identify its possible roles in testis development and sperm motility regulation.The mRNA and protein expression of SOX5 in the testis of roosters at 0,5,15,40 and 60 weeks of age were determined by RT-qPCR and Western blot,respectively.Its cellular localization in the testis was determined by immunohistochemistry.The expression of SOX5 in the testis of normal and asthenospermia roosters at 40 weeks of age was also compared.The results showed that SOX5 mRNA and protein were differentially expressed in the testis at different ages (P＜0.05).The expression pattern of SOX5 protein was in accordance with the mRNA.The immunohistochemical staining revealed that SOX5 was expressed in the Sertoli cells and spermatogonia at 0 and 5 weeks of age.SOX5 was high-expressed in Sertoli cells,spermatocytes,secondary spermatocytes,round and elongated spermatids,and mature sperm in the testis at 15 weeks of age.The expression started to decrease at 40 weeks.The expression pattern of SOX5 was in accordance with the sexual development and recession of the roosters,i.e.high-expressed around the sexual maturation and reproduction peak and low-expressed before the puberty and sexual recession.In addition,the expression of SOX5 in the asthenospermia roosters was lower than the normal ones at 40 weeks of age (P＜0.05).In conclusion,this study indicated that the expression of SOX5 was associated with testis development and sperm motility in chickens,suggesting its regulatory roles in these processes.关键词
公鸡/SOX5/睾丸/精子活力/精子发生Key words
roosters/SOX5/testis/sperm motility/spermatogenesis分类
农业科技引用本文复制引用
许红,王梁,陈继兰,孙研研,石雷,刘一帆,白皓,李云雷,黄子妍,叶建华,贾亚雄..SOX5蛋白对公鸡精子发生和精子活力的作用及其定位[J].畜牧兽医学报,2018,49(4):718-724,7.基金项目
国家自然科学基金(31372304 （）
31672406) （）
现代农业产业技术体系(CARS-40) （CARS-40）
中国农业科学院科技创新工程(ASTIP-IAS04 （）
CAAS-XTCX2016010-03) （）
