畜牧兽医学报2018，Vol.49Issue(4)：736-745,10.DOI:10.11843/j.issn.0366-6964.2018.04.010
复合菌剂发酵玉米秸秆对绵羊瘤胃液细菌多样性的影响
Effect of Corn Stalk Fermented by Complex Bacterial on Rumen Bacteria Diversity in Sheep
摘要
Abstract
The present study was conducted to investigate the effects of fermented corn stalk on rumen bacterial diversity of sheep via 16S rDNA high-throughput sequencing.Twelve F1 generation mature rams of South African Merino sheep ♂ × Northeast China Fine-fleece sheep♀ with rumen fistula were divided into two groups randomly and fed with corn stalk silage and fermented corn stalk,respectively.Rumen fluid was collected at different time points as following:1 day before feeding,6 h after the 7th and 14th day morning feeding.Samples collected at the same time point in the same group were mixed well and analyzed for bacterial diversity by highthroughput sequencing,which were marked as:CS group(YD0,YD7,YD21) from the corn stalk silage group and FCS group(YS0,YS7,YS21) from the fermented corn stalk group.The results showed that:1)The bacterial community in collected rumen fluid was consisted of 29 phyla,74 classes,135 orders,215 families and 428 genera.2) The dominant bacteria were identified as Bacteroidetes followed by Lentisphaerae,Firmicutes and Fibrobacteres with successively decreased amount in the FCS group,while successively Bacteroidetes,Firmicutes,Lentisphaerae and Fibrobacteres in the CS group.In addition,the abundance of Bacteroidetes and Firmicutes in CS group were lower compared with FCS group.The abundance of Lentisphaerae and Fibrobacteres increased in both groups,while a larger increase in CS group after 21 days feeding.3)Prevotella was found to be the dominant genera in YS7 (15.56％),however its abundance reduced dramatically in CS group(from 10.48％ to 3.17％) after 7 days feeding;the increase in abundance of Fibrobacter was higher in the CS group than that in FCS group after 21 days feeding.4)Furthermore,with 97 similarity,the Shannon and Simpson indexes of YS21 in the FCS group were higher than that of YD21 in the CS group,indicating the higher bacterial community diversity.In conclusion,the fermented corn stalk with complex bacteria increased the rumen bacteria diversity.The ratio of Bacteroidetes and Firmicutes in sheep rumen reduced,while the populations of Lentisphaerae and Fibrobacteres increased in the FCS diet.关键词
发酵玉米秸秆/绵羊/瘤胃细菌/多样性Key words
fermented corn stalk/sheep/rumen bacteria/diversity分类
农业科技引用本文复制引用
郭威,郭晓军,周贤,李术娜,朱宝成..复合菌剂发酵玉米秸秆对绵羊瘤胃液细菌多样性的影响[J].畜牧兽医学报,2018,49(4):736-745,10.基金项目
河北省重点研发计划农业关键共性技术攻关专项(16226604D) （16226604D）
河北省技术创新引导计划科技型中小企业技术创新资金专项(15C1303121015) （15C1303121015）
河北省畜牧兽医局科技项目(2015-3-01) （2015-3-01）
