畜牧兽医学报2018，Vol.49Issue(4)：754-760,7.DOI:10.11843/j.issn.0366-6964.2018.04.012
K亚群禽白血病病毒5'LTR序列及启动活性分析
The Research of 5'LTR Promoter Activity of Avian Leukosis Virus Subgroup K
摘要
Abstract
In this study,we investigated the proliferation of avian leukosis virus subgroup K (ALV-K) and the activity of LTR.An ALV-K strain was used to infect DF-1 cells and the LTR was cloned into pGL3-Basic vector.The replication ability of the virus on ALV susceptible cell DF-1 was evaluated by ALV p27 antigen ELISA,and the promoter activities of the LTR of ALV-K,ALV-E and ALV-J strains were measured using a Dual-Glo Luciferase Assay System.The results showed that promoter activity of ALV-K LTR was significantly lower than those of other exogenous ALV in DF-1,CEF and 293T cells.The relatively poorer replication ability of ALV-K may be related to its weak promoter activity of the LTR.关键词
ALV-K/致病性/LTR/启动活性Key words
ALV-K/pathogenicity/LTR/promoter activity分类
农业科技引用本文复制引用
赵子君,饶明章,陈建,张杰,袁丽霞,廖明,曹伟胜..K亚群禽白血病病毒5'LTR序列及启动活性分析[J].畜牧兽医学报,2018,49(4):754-760,7.基金项目
国家自然科学基金(31672552) （31672552）
广东省自然科学基金(2016A030313403) （2016A030313403）
广东省家禽产业技术体系疾病控制岗位专家(2016LM1114) （2016LM1114）
国家肉鸡产业技术体系(CARS-41-G16) （CARS-41-G16）
