畜牧兽医学报2018，Vol.49Issue(4)：794-803,10.DOI:10.11843/j.issn.0366-6964.2018.04.017
人工感染沙门菌牦牛脾中相关差异表达基因筛选
Screening of Differentially Expressed Genes Associated with Salmonella Infection from Spleen of Artificially Infected Yak
摘要
Abstract
The present study was designed to analyze the immune genes against Salmonella infection and their regulatory network in yak at the genomic transcriptional level.Using yak Salmonella as the model pathogen and yak as the object,transcriptome sequencing was performed on the central immune organ of spleen in infected yak by RNA-Seq sequencing technology at three different periods of 12,24,and 48 hours,respectively.Transcriptome data from the three different periods were compared between the infected and the control healthy yaks and differentially expressed genes were screened out.The GO and KEGG functional analysis of these differentially expressed genes were conducted and the co-expression network was further analyzed.Results showed that 413 differentially expressed genes were selected,of which 185 were up-regulated and 228 were down regulated.GO analysis showed that genes related to biological process accountedfor the largest percentage,among which the most enriched ones were those involved in cellular process,metabolic process,biological regulation,regulation of biological process and single organism process.KEGG analysis revealed that infection-and immune-related pathways presented a larger portion in the top 20 pathways enriched in all three periods,and the major genes involved in these pathways were chemokines.WGCNA analysis showed 66 genes in network hubs,and those in the network center were SCOC and NOCT.The present study explored the molecular interaction mechanism of spleen cells against Salmonella in yak at the omics level.关键词
牦牛/沙门菌/脾/转录组/差异基因/RNA-SeqKey words
yak/Salmonella/spleen/transcriptome/differential genes/RNA-Seq分类
农业科技引用本文复制引用
陈通,朱育星,蔡雯袆,杨琦玥,邓志和,兰道亮..人工感染沙门菌牦牛脾中相关差异表达基因筛选[J].畜牧兽医学报,2018,49(4):794-803,10.基金项目
四川省青年科技基金项目(2014JQ0043) （2014JQ0043）
西南民族大学中央高校基本科研业务费专项资金(2015NZYTD01) （2015NZYTD01）
