畜牧兽医学报2018，Vol.49Issue(4)：804-810,7.DOI:10.11843/j.issn.0366-6964.2018.04.018
捻转血矛线虫半胱氨酸蛋白酶对山羊PBMCs免疫功能的影响
Effects of Haemonchus contortus Cysteine Protease on Immune Functions of Goat PBMCs
摘要
Abstract
The present study was designed to investigate the effects of Haemonchus contortus Cysteine protease on immune functions of peripheral blood mononuclear cells (PBMCs) of goat.PBMCs were separated from goat blood sample,monocytes were isolated from PBMCs because of their adherent growth.Recombinant protein of different concentrations (0,10,20,40μg · mL-1) was cultured with PBMCs or monocytes in vitro.Nitric oxide (NO) secretion was measured by nitrate reductase assay.Cell migration was tested by Cell Culture Inserts.The mRNA transcriptional levels of IL-2,IL-4,IL-10,IL-17,IFN-γ and TGF-β were detected by real-time fluorescent quantitative PCR.Then cell phagocytosis and apoptosis were calculated by flow cytometry.The result revealed that Hc58 was able to dramatically enhance NO release and phagocytosis of monocytes.Hc58 might significantly up-regulate the production of IL-2,IL-4,IL-17 and IFN-γ.Furthermore,it also could notably increase apoptosis ratio and migration of PBMCs.There were signs that Haemonchus contortus Cysteine protease impacted on the immune functions of goat PBMCs via different pathways.关键词
捻转血矛线虫/半胱氨酸蛋白酶/山羊/外周血单个核细胞/流式细胞术Key words
Haemonchus contortus/cysteine protease (Hc58)/goat/peripheral blood mononuclear cells/flow cytometry分类
农业科技引用本文复制引用
胡孟娟,周丽娜,牛延萍,徐立新,宋小凯,李祥瑞,严若峰..捻转血矛线虫半胱氨酸蛋白酶对山羊PBMCs免疫功能的影响[J].畜牧兽医学报,2018,49(4):804-810,7.基金项目
中央高校基本科研业务费(KYYJ201710) （KYYJ201710）
国家重点基础发展研究计划(973计划)(2015CB150300) （973计划）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
