中国临床药理学杂志2018，Vol.34Issue(4)：439-442,4.DOI:10.13699/j.cnki.1001-6821.2018.04.013
长柱重楼总皂苷体外抗肿瘤活性及毒性研究
Antitumor activity in vitro and toxicity of the total saponins from Paris forrestii
摘要
Abstract
Objective To study the antitumor activity in vitro and toxicity of the total saponins from Paris forrestii (PCT3).Methods Inhibitory effect of PCT3 on the proliferation of human lung cancer (A-549) and human hepatoma cells (HEPG-2) was measured via 3-(4,5-dimethylthiazol-2yl)-2,5-diphenylt etrazoliumbromide assay;PCT3 was injected at the dose of 52,74,105,150 mg· kg-1 to detect the half lethal dose (LD50);Hemolytic effect of PCT3 was determined by colorimetric method.Results The 50％ inhibitory concentration (IC50) values of PCT3 were 11.71 μg· mL-1 and 2.36 μg · mL-1 on A-549 and HEPG-2 cells,respectively.The IC50 values of cisplatin were 6.31 μg · mL-1 and 5.33 μg · mL-1 on A-549 and HEPG-2 cells,respectively.LD50 of PCT3 was 92.40 mg · kg-1,and it showed a certain hepatic toxicity and certain inhibitory effects on overall health status of the animal;The hemolytic activity of PCT3 was dose dependent,and the half effective dose (ED50) was 4.31 μg · mL-1.Conclusion PCT3 had obvious inhibitory effect on the proliferation of A-549 and HEPG-2 cell lines,the later is stronger than the former.Acute toxicity test in mice showed that PCT3 had a certain toxicity;PCT3 had remarkable hemolytic activity,there was a significant correlation between hemolytic activity and cytotoxic activity.关键词
长柱重楼/总皂苷/体外抗肿瘤活性/急性毒性/溶血作用Key words
Paris forrestii/total saponin/antitumor activity in vitro/acute toxicity/hemocytolysis分类
医药卫生引用本文复制引用
杨蓉蓉,王跃虎,施敏,陈雪梅,陈英杰,王鹏,周轶平..长柱重楼总皂苷体外抗肿瘤活性及毒性研究[J].中国临床药理学杂志,2018,34(4):439-442,4.基金项目
云南省教育厅科学研究基金重点基金资助项目(2014Z060) （2014Z060）
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2014FB011) （2014FB011）
