摘要
Abstract
Objective To investigate the inhibitory effect of cisplatin (DDP) combined with esophageal cancer associated gene 2 (ECRG2) on the proliferation of human esophageal carcinoma EC9706 cells and its mechanism.Methods Human esophageal carcinoma EC9706 cells were divided into blank control group,positive control group and low,medium and high concentration experimental groups.Positive control group cells were treated with 3 mg · L-1 cisplatin.The cells in low,medium and high concentration groups were treated with 5.5,7.5 and 9.5 μg · L-1 ECRG2,blank control group cells was given no treatment.The activity and apoptosis rate of cells in each group were detected,and the expression of multi-drug resistance gene in human esophageal carcinoma cell line EC9706 was detected by reverse transcriptase-polymerase chain reaction (RT-PCR).Results The cell viability of EC9706 cells in control group,positive control group and low,medium and high concentration experimental groups were 100.00％,(92.12 ±2.36)％,(88.62 ±3.22)％ and (82.15 ±2.05)％,(78.41 ±2.16)％.The cell viability in positive control group and three experimental groups were significantly lower than that in blank control group (P ＜ 0.05 or P ＜ 0.01),and those in medium and high concentration groups were significantly lower than that in positive control group (P ＜ 0.05).The cell viability in medium and high concentration groups decreased with the increasing of ECRG2 protein concentration (P＜0.01).The apoptotic rates of EC9706 cells in positive control group,low,medium and high concentration experimental groups were (5.21 ± 0.24) ％,(11.45 ± 0.66) ％,(16.86-± 1.42) ％ and (22.62 ± 2.12) ％,had significant difference with that in blank control group,which was (2.89 ± 0.32) ％.The apoptotic rates of low,medium and high concentration experimental groups were significantly higher than that in pos-itive control group (P ＜ 0.01).The relative expression levels of GST-π and MRP mRNA in positive control group and low,medium and high concentration experimental groups were lower than those in blank control group,the low,medium and high concentrations experimental groups were lower than positive control group (P ＜ 0.05 or P＜0.01).Conclusion Cisplatin can enhance the inhibitory effect of ECRG2 on the proliferation of human esophageal carcinoma EC9706 cells and promote apoptosis.The mechanism may be related to the significant decrease of GST-π and MRP gene expression by cisplatin combined with ECRG2.关键词
食管癌/顺铂/食管癌相关基因2/增殖/凋亡Key words
esophageal cancer/cisplatin/esophageal cancer related gene 2/proliferation/apoptosis分类
医药卫生
