中国临床药理学杂志2018，Vol.34Issue(4)：464-466,3.DOI:10.13699/j.cnki.1001-6821.2018.04.020
反相高效液相色谱法测定人血浆中紫杉醇浓度
Determination of paclitaxel in human plasma by reversed-phase high performance liquid chromatography
摘要
Abstract
Objective To establish a reversed-phase high performance liquid chromatography method to determine the paclitaxel concentration in human plasma.Methods The chromatographic column was InertSustain C18 (250 mm× 4.6 mm,5 μm).The mobile phase was consisted of methanol-acetonitrile-water (30 ∶ 30 ∶ 40) at the flow rate of 1.0 mL · min-1 The ultraviolet detection wavelength was 230 nm and the column temperature was 35 ℃.The plasma samples were extracted by the method of liquid-liquid extraction with methyl tert-butyl ether,and diazepam was used as the internal standard.The specificity,precision,recovery,stability and reproducibility were analyzed.Results Calibration curves were linear over the concentration range of 2.75 × 10-1-11.00 μg · mL-1.The recovery rate was 96.28％ to 97.85％,and the extraction recovery was 85.36％-90.83％.The intra-day and inter-day RSD were both less than 2.84％.Paclitaxel in human plasma was stable after storage at-20 ℃ in 15 days or freeze-thaw for three times.Conclusion The method is simple,rapid and repeatable,with little quantity of sample and high recovery rate.It can be used to determine the paclitaxel concentration in the plasma of tumor patients.关键词
紫杉醇/高效液相色谱法/血药浓度Key words
paclitaxel/high performance liquid chromatography/plasma concentration分类
医药卫生引用本文复制引用
许秋霞,庄奕筠,高雅莉,吴冰冰,张吟..反相高效液相色谱法测定人血浆中紫杉醇浓度[J].中国临床药理学杂志,2018,34(4):464-466,3.基金项目
福建省科技厅社会发展引导性基金资助项目(2015Y0055) （2015Y0055）
福建省卫计委青年科研课题基金资助项目(2015-1-60) （2015-1-60）
泉州市医疗卫生指导性基金资助项目(2015QZZD13) （2015QZZD13）
福建医科大学附属第二医院苗圃基金资助项目(2013MP05) （2013MP05）
