中国实验动物学报2018，Vol.26Issue(2)：139-144,6.DOI:10.3969/j.issn.1005-4847.2018.02.001
C57BL/6 J小鼠黑色素瘤肺转移模型的构建
Establishment of a C57BL/6 J mouse model of metastatic melanoma in the lung
摘要
Abstract
Objective To investigate the influencing factors involved in the establishment of a C57BL/6 J model of metastatic melanoma in the lung,including the way of tumor inoculation,the number of inoculated cells and the time of tumor formation. Methods Mouse melanoma B16F10 cells were cultured in vitro. 1)Eighteen healthy male C57BL/6 J mice were randomly divided into three groups. Mice in each group received 100 μL cell suspension(including 3 ×106 melanoma cells)via intravenous,intraperitoneal and subcutaneous injection,respectively. After two weeks,the mice were killed and dissected,and the tumor growth and metastasis were observed. 2)Eighteen male mice were randomly divided into three groups. Mice in each group were injected with 3 ×106cells,1 ×106cells, and 3 ×105cells through the tail vein,respectively. After two weeks,mice were killed and dissected,and the tumor growth and metastasis were observed. 3)Eighteen male mice were randomly divided into three groups. Mice in each group were injected with 1×106cells though the tail vein. Mice were killed and dissected after one week, two weeks and three weeks, respectively. The growth and metastasis of tumor were observed. Results 1)The success rate of lung metastasis was 100% in the mice with intravenous injection,but not in mice receiving intraperitoneal injection and subcutaneous injection. 2)The size of metastatic melanoma nodules were moderate in mice inoculated by 1 ×106cells. The number of melanoma metastatic foci was too high in the mice inoculated with 3 ×106cells,but too low in the mice inoculated with 3 ×105cells. 3)Significant metastatic melanoma foci were observed in the mice killed and dissected after two weeks with no death. The number of melanoma foci in the lung was too high in the mice killed after three weeks,while was too low in the mice killed at one week after tumor cell inoculation. Conclusions Intravenous injection of 1×106mouse melanoma cells into C57BL/6 J mice and killed after two weeks is an optimal method for establishment of a mouse model of metastatic melanoma in the lung, and is worth of recommendation.关键词
C57BL/6J小鼠/小鼠黑色素瘤细胞B16F10/肺转移/肿瘤模型Key words
C57BL/6 J mice/mouse melanoma cell line B16F10/lung metastasis/tumor model分类
生物科学引用本文复制引用
孟星君,李孝东,刘俊,周康熙,崔庆亚,胡仁萍,闫荣,戴克胜..C57BL/6 J小鼠黑色素瘤肺转移模型的构建[J].中国实验动物学报,2018,26(2):139-144,6.基金项目
国家自然科学基金重点项目(No.81130008) （No.81130008）
江苏省普通高校学术学位研究生创新计划项目.Funded by National Natural Science Foundation of China(No.81130008) （No.81130008）
Innovation Plan Project of Academic Degree Graduate Students in Jiangsu General Colleges and Universities. （）
