中国实验动物学报2018，Vol.26Issue(2)：145-149,5.DOI:10.3969/j.issn.1005-4847.2018.02.002
硫辛酸合成酶在Leprdb/db小鼠肝肾中的表达
Effect of leptin on expression of lipoic acid synthase in the liver and kidney of Leprdb/dbmice
摘要
Abstract
Objective To study the expression of lipoic acid synthase(LIAS)in the liver and kidney of Leprdb/db mice with deficient leptin receptor. Methods Eight 10-week old male Leprdb/ +mice and Leprdb/dbmice were included in this study. The body weight of rats in the two groups was measured. Fasting blood glucose(FPG)was measured with blood glucose test strips for all mice after fasting for 8 hours. Blood samples were obtained from the abdominal aorta and the animals were sacrificed. The liver and kidney were weighed. The right lobe of liver and the left kidney samples were fixed in 4% paraformaldehyde for pathological examination. Serum samples were separated and the sereum contents of CHO, TG,HDL and LDL were detected. The mitochondria of liver and kidney tissues were extracted with a mitochondrial isolation kit, and the protein was extracted. The expression of LIAS protein was detected by western blot. Results Histopathological observation showed that the liver and kidney tissues of Leprdb/ +mice have intact and clear structure. But the liver tissue of Leprdb/dbmice showed fatty degeneration, the kidney tissue showed glomerular hypertrophy, basement membrane thickening, mesangial area widened, including mesangial cells and mesangial matrix increased. The GLU,CHO,TG,LDL and AST of Leprdb/dbmice were significantly increased compared with those of Leprdb/ +mice(P<0.05). Compared with Leprdb/ +mice,the LIAS protein expression was significantly increased in the liver and kidney mitochondria of Leprdb/dbmice(P<0.05). Conclusions There is impaired glucose and lipid metabolism in the Leprdb/dbmice which has defect leptin receptor,and the expression of LIAS protein in liver and kidney of the Leprdb/dbmice is higher than that of Leprdb/ +mice.关键词
Leprdb/db小鼠/硫辛酸/肝/肾/瘦素Key words
Leprdb/dbmice/lipoic acid/liver/kidney/leptin分类
生物科学引用本文复制引用
彭强,赵英政,闫婷婷,翟晓楠,张旭旭,易宪文,张合喜,徐光翠..硫辛酸合成酶在Leprdb/db小鼠肝肾中的表达[J].中国实验动物学报,2018,26(2):145-149,5.基金项目
国家自然科学基金面上项目(No.81370916) （No.81370916）
国家自然科学基金青年基金(No.81703183) （No.81703183）
河南省科技厅科技攻关项目(No.182102310078).Funded by National Natural Science Foundation of China(No.81370916),funded by the National Natural Science Foundation of China(No.81703183),Funded by Scientific and technological research project of Henan provincial science and Technology Department(182102310078). （No.182102310078）
