中国实验动物学报2018，Vol.26Issue(2)：158-164,7.DOI:10.3969/j.issn.1005-4847.2018.02.004
中国地鼠口腔颊黏膜癌中miRNA与mRNA表达谱的关联分析
Association of expression profiles of miRNA and mRNA in Chinese hamster buccal pouch squamous cell carcinoma
摘要
Abstract
Objective To establish the expression profiles of differentially expressed miRNA and mRNA in oral squamous cell carcinoma(OSCC)and to investigate the roles of miRNA and mRNA associated with the occurrence and development of OSCC. Methods The expression profiles of miRNA and mRNA were constructed using a new generation of high-throughput sequencing techniques. The miRNA and mRNA associated with the occurrence and development of OSCC were predicted by Gene Ontology enrichment analysis and KEGG pathway analysis. Results We successfully constructed the differentially expressed miRNA and mRNA profiles of Chinese hamster buccal pouch squamous cell carcinoma. 11 known and 3 novel significantly differentially expressed miRNAs and 194 differentially expressed mRNAs were found. A miRNA can regulate multiple mRNAs, and multiple miRNAs can control one mRNA. Conclusions Differential expression of miRNA play a an important role in the carcinogenesis and development of OSCC through regulating mRNA and forming a complex regulatory network. It provides theoretical data for the occurrence,pathogenesis,clinical treatment and prognosis of OSCC.关键词
口腔鳞状细胞癌/miRNA/mRNA/高通量测序Key words
oral squamous cell carcinoma/OSCC/miRNA/mRNA/high-throughput sequencing分类
生物科学引用本文复制引用
李莉红,庞文彪,常凯,阎小艳,高继萍,宋国华..中国地鼠口腔颊黏膜癌中miRNA与mRNA表达谱的关联分析[J].中国实验动物学报,2018,26(2):158-164,7.基金项目
国家自然科学基金(No.31772551),山西省自然科学基金(No.201701D121087),山西省研究生教育创新项目(No.2017SY037).Funded by National Natural Science Foundation of China(No.31772551),Natural Science Foundation of Shanxi Province(No.201701D121087)and Postgraduate Education Innovation Project of Shanxi Province(No.2017SY037). （No.31772551）
