中国实验动物学报2018，Vol.26Issue(2)：174-180,7.DOI:10.3969/j.issn.1005-4847.2018.02.006
维吾尔医异常黑胆质证大鼠模型的尿液代谢组研究
Urine metabonomics study of abnormal Savda syndrome rat model in Uyghur traditional medicine
摘要
Abstract
Objective To evaluate and discuss the changes of biomarkers of abnormal Savda syndrome rat model in Uyghur traditional medicine by external feature observation and urine metabolomics assessment. Methods The abnormal Savda syndrome rat model was established according to the theory of Uyghur traditional medicine. Its external characteristics such as hair, tongue, sleep, feces, emotion and weight growth rate were observed and scored, and their urine was detected and analyzed by proton nuclear magnetic resonance(1H-NMR)spectroscopy. Results Compared with the healthy control group,there were significant changes in the external features in the abnormal Savda syndrome rat model group,including dry and hard stools,reduced urine output and darker color,dry fur,dark purple tongue with ecchymosis, and decreased weight growth rate. Moreover,23 urinary metabolites were significantly reduced,including propionate,lactic acid, pyruvic acid, acetic acid, alanine, acetamide, glycoprotein, acetone, methyl guanidine, sarcosine, ornithine, glycine, creatine, creatinine, aminoanhydride, β-galactose, urocanate, tyrosine, phenylalanine, hippuric acid, aminohippuric acid,formic acid and lysine. However,urea,citric acid,allantoin and α-ketoglutaric acid were significantly increased. Conclusions During the development process of Savda syndrome, there are not only abnormal changes in external appearance in the model rats, but also evident changes of many internal metabolic pathways. The obvious abnormalities of the urine metabolites may be related to the biological mechanisms of abnormal Savda syndrome.关键词
异常黑胆质/大鼠模型/代谢组学Key words
abnormal Savda syndrome/rat model/metabonomics分类
生物科学引用本文复制引用
米热阿依·亚力昆,阿合尔扎马尼·麦麦提,巴哈古力·阿卜都热合曼,太力艾提·吐尔洪,迪那拉·恰热甫汗,阿仙姑·哈斯木,巴吐尔·买买提明..维吾尔医异常黑胆质证大鼠模型的尿液代谢组研究[J].中国实验动物学报,2018,26(2):174-180,7.基金项目
国家自然科学基金(No.81460749) （No.81460749）
国家自然科学-新疆联合基金(No.U1603119).Funded by National Natural Science Foundation of China(No.81460749)and National Natural Science-XINJIANG United Foundation(No.U1603119). （No.U1603119）
