中国实验动物学报2018，Vol.26Issue(2)：188-194,7.DOI:10.3969/j.issn.1005-4847.2018.02.008
实验室饲养和野外生存条件下东方田鼠肠道菌群的多样性
Comparative analysis of intestinal flora of Microtus fortis living under laboratory feeding and wild survival conditions
摘要
Abstract
Objective To compare the differences of bacterial distribution of intestinal flora in Microtus fortis living under laboratory feeding and wild survival conditions. Methods The 16S rDNA-V4-V5 region of bacteria in the ileocecal contents from Microtus fortis raised in lab and captured in wild were measured by high-throughput sequencing. The number of operational taxonomic units(OTUs)were sorted and calculated,and the species abundance and distribution and difference were analyzed. Results The rarefaction curves indicated that adequate sampling was achieved. At the phylum level,the distribution of intestinal flora between two groups was similar. The experimental group had a unique phylum, Lentisphaerae. The wild type group had 3 unique phylums,Fusobacteria,Thaumarchaeota and an unclassified phylum. At the genus level, the kind of intestinal flora in the wild type group was more abundant than the experimental group. Ruminococcus is the largest differential genus. Conclusions The microbial community structure and differences of Microtus fortis living under different conditions are obtained. It may further enrich the basic biology data of Microtus fortis.关键词
东方田鼠/肠道菌群/16SrDNA/多样性Key words
Microtus fortis/intestinal flora/16S rDNA/bacterial diversity分类
生物科学引用本文复制引用
冯洁,沈志敏,王胜昌,林金杏,柏熊,谢建云..实验室饲养和野外生存条件下东方田鼠肠道菌群的多样性[J].中国实验动物学报,2018,26(2):188-194,7.基金项目
国家科技支撑计划(No.2015BAI09B04).Funded by National Science and Technology Support Program(No.2015BAI09B04). （No.2015BAI09B04）
