中国实验动物学报2018，Vol.26Issue(2)：195-200,6.DOI:10.3969/j.issn.1005-4847.2018.02.009
系统性红斑狼疮小鼠肠道微生物与抗dsDNA抗体水平的相关性
Correlation between gut microbiota and anti-dsDNA antibody in the mouse model of systemic lupus erythematosus
摘要
Abstract
Objective To explore the relationship between gut microbiota and anti-dsDNA antibody in systemic lupus erythematosus(lupus)TC mice. Methods ELISA was performed to detect serum anti-dsDNA antibody in the lupus TC mice. Then,the feces of both dsDNA-positive and -negative mice were collected, and 16S rRNA of the stool sample was sequenced by Illumina HiSeq 2500 high-throughput sequencing,to analyze the relationship between gut microbiota and anti-dsDNA antibody level in the two groups. Results The result of ELISA and mouse fecal high-throughput sequencing showed that the species diversity of gut microbiota in the dsDNA-positive TC mice was significantly lower than that in the dsDNA-negative TC mice. The gut microbiota of TC mice in the two groups showed significant differences at different classification levels: Chloroflexi at phylum level, Betaproteobacteria, Deltaproteobacteria and Ktedonobacteria at class level,Burkholderiales,Desulfovibrionales and Ktedonobacterales at order level,Alcaligenaceae and Desulfovibrionaceae at family level,and Parasutterella and Desulfovibrio at genus level(P<0.05 for all). Conclusions The flora composition of gut microbiota in lupus TC mice is highly correlated with anti-dsDNA antibody. The results of our study provide a basis for further elucidation of the relationship between gut microbiota and the pathogenetic mechanism of systemic lupus erythematosus.关键词
肠道微生物菌群/系统性红斑狼疮/抗dsDNA抗体Key words
gut microbiota/systemic lupus erythematosus/Anti-dsDNA antibody/mice分类
生物科学引用本文复制引用
邹桂香,段新旺,牛海涛..系统性红斑狼疮小鼠肠道微生物与抗dsDNA抗体水平的相关性[J].中国实验动物学报,2018,26(2):195-200,6.基金项目
国家重点研发计划资助(No.2017YFC1103603) （No.2017YFC1103603）
中国医学科学院医学与健康科技创新工程(No.2016-12M-1-006).Funded by National Key R&D Program of China(No.81371384)and CAMS Initiative for Innovative Medicine(No.2016-12M-1-006). （No.2016-12M-1-006）
