中国比较医学杂志2018，Vol.28Issue(4)：1-6,6.DOI:10.3969/j.issn.1671-7856.2018.04.001
FKBP51参与高脂诱导的线粒体损伤
Involvement of FKBP51 in mitochondrial damage induced by high fat diet feeding
摘要
Abstract
Objective To investigate the effect of high fat diet feeding on mitochondrial structure and function. Methods Male C57BL/6J mice at the age of 4 weeks were used in this study. After 6 weeks of regular diet(RD)or high-fat diet(HF)feeding, the high fat-induced obesity phenotype was confirmed by body weight measurement and liver histopathology. RNA was isolated from the liver tissue of RD and HF mice and the expression profiles were detected using RNA-seq. Differentially expressed genes between RD and HF mice were analyzed using BRB-ArrayTools. DAVID online tools were applied to analyze the GO and KEGG pathways. Transmission electron microscopy and western blotting were performed to observe the mitochondrial ultrastructure and quantified the expression of function-related proteins. Results Compared with the RD mice,the body weight gain was faster in the HF mice. The size of the lipid droplets was bigger in the HF-fed mouse liver tissue. Multiple pathway analysis all identified that these major gene changes were related to mitochondria. The mitochondrial deformation,enlarged or even destruction was observed in the high fat diet group observed by transmission electron microscopy. This observation was further confirmed by detecting of the expression of genes in the HF liver mitochondria. The levels of MFN1 and PHB1 were significantly increased, while the level of FKBP51 was significantly decreased. Conclusions FKBP51 is involved in the high-fat-induced mitochondrial damage via morphological and structural damages of mitochondria.关键词
FKBP51/线粒体损伤/高脂Key words
FKBP51/mitochondrial damage/high fat diet/mice分类
医药卫生引用本文复制引用
刘明,邱彬,王婷婷,邓然,刘云波,雍伟东..FKBP51参与高脂诱导的线粒体损伤[J].中国比较医学杂志,2018,28(4):1-6,6.基金项目
中国医学科学院医学创新基金(DCIFM 2017-I2M-3-015) （DCIFM 2017-I2M-3-015）
国家自然科学基金(81700751,81272273) （81700751,81272273）
北京市自然科学基金(7164278). （7164278）
